Abstract
Wood chips of Pinus radiata softwood were biotreated with the brown rot fungus (BRF) Gloeophyllum trabeum for periods from 4 and 12 weeks. Biodegradation by BRF leads to an increase in cellulose depolymerization with increasing incubation time. As a result, the intrinsic viscosity of holocellulose decreased from 1,487 cm3/g in control samples to 783 and 600 cm3/g in 4- and 12-week decayed wood chips, respectively. Wood weight and glucan losses varied from 6 to 14% and 9 to 21%, respectively. Undecayed and 4-week decayed wood chips were delignified by alkaline (NaOH solution) or organosolv (ethanol/water) processes to produced cellulosic pulps. For both process, pulp yield was 5–10% lower for decayed samples than for control pulps. However, organosolv bio-pulps presented low residual lignin amount and high glucan retention. Chemical pulps and milled wood from undecayed and 4-week decayed wood chips were pre-saccharified with cellulases for 24 h at 50°C followed by simultaneous saccharification and fermentation (SSF) with the yeast Saccharomyces cerevisiae IR2-9a at 40°C for 96 h for bioethanol production. Considering glucan losses during wood decay and conversion yields from chemical pulping and SSF processes, no gains in ethanol production were obtained from the combination of BRF with alkaline delignification; however, the combination of BRF and organosolv processes resulted in a calculated production of 210 mL ethanol/kg wood or 72% of the maximum theoretically possible from that pretreatment, which was the best result obtained in the present study.
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References
Araque E, Parra C, Freer J, Contreras D, Rodríguez J, Mendonça R, Baeza J (2008) Evaluation of organosolv pretreatment for the conversion of Pinus radiata D. Don to ethanol. Enzyme Microb Technol 43:214–219
Claassen PAM, van Lier JB, Contreras AML, van Niel EWJ, Sijtsma L, Stams AJM, de Vries SS, Weusthuis RA (1999) Utilisation of biomass for the supply of energy carriers. Appl Microbiol Biotechnol 52:741–755
Ferraz A, Mendonça R, Silva FT (2000) Organosolv delignification of white- and brown-rotted Eucalyptus grandis hardwood. J Chem Technol Biotechnol 75:18–24
Filley TR, Cody GD, Goodell B, Jellison J, Noser C, Ostrofsky A (2002) Lignin demethylation and polysaccharide decomposition in spruce sapwood degraded by brown rot fungi. Org Geochem 33:111–124
Galbe M, Zacchi G (2002) A review of the production of ethanol from softwood. Appl Microbiol Biotechnol 59:618–628
Green F, Highley TL (1997) Mechanism of brown-rot decay: paradigm or paradox. Int Biodeterior Biodegradation 39:113–124
Goodell B (2003) Brown-rot fungal degradation of wood: our evolving view. In: Goodell B, Schultz T, Nichols D (eds) Wood deterioration and preservation-advances in our changing world. ACS Symp Ser 845:97–118
Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69:627–642
Mendonça R, Guerra A, Ferraz A (2002) Delignification of Pinus taeda wood chips for preparing high-yield kraft pulps from Ceriporiopsis subvermispora-biotreated samples. J Chem Technol Biotechnol 77:411–418
Mendonça RT, Jara JF, González V, Elissetche JP, Freer J (2008) Evaluation of the white-rot fungi Ganoderma australe and Ceriporiopsis subvermispora in biotechnological applications. J Ind Microbiol Biotechnol 35:1323–1330
Muñoz C, Mendonça R, Baeza J, Berlin A, Saddler J, Freer J (2007) Bioethanol production from bio-organosolv pulps of Pinus radiata and Acacia dealbata. J Chem Technol Biotechnol 82:767–774
Pan X, Arato C, Gilkes N, Gregg D, Mabee W, Pye K, Xiao Z, Zhang X, Saddler J (2005) Biorefining of softwoods using ethanol organosolv pulping: preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. Biotechnol Bioeng 90:473–481
Pan X, Kadla JF, Ehara K, Gilkes N, Saddler J (2006) Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions, and antioxidant activity. J Agric Food Chem 54:5806–5813
Sarkanen KV (1990) Chemistry of solvent pulping. Tappi J 73:215–219
Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11
Suzuki MR, Hunt CG, Houtman CJ, Dalebroux ZD, Hammel KE (2006) Fungal hydroquinones contribute to brown rot of wood. Environ Microbiol 8:2214–2223
Taherzadeh MJ, Eklund R, Gustafsson L, Niklasson C, Liden G (1997) Characterization and fermentation of dilute-acid hydrolyzates from wood. Ind Eng Chem Res 36:4659–4665
Taherzadeh M, Karimi K (2007) Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials: a review. Bioresources 2:707–738
Wingren A, Galbe M, Zacchi G (2003) Techno-economic evaluation of producing ethanol from softwood: comparison of SSF and SHF and identification of bottlenecks. Biotechnol Progr 19:1109–1117
Yokoyama T, Kadla JF, Chang H-m (2002) Microanalytical method for the characterization of fiber components and morphology of woody plants. J Agric Food Chem 50:1040–1044
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Financial support from FONDECYT (grants 1050941 and 1080303) is acknowledged.
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Fissore, A., Carrasco, L., Reyes, P. et al. Evaluation of a combined brown rot decay–chemical delignification process as a pretreatment for bioethanol production from Pinus radiata wood chips. J Ind Microbiol Biotechnol 37, 893–900 (2010). https://doi.org/10.1007/s10295-010-0736-3
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DOI: https://doi.org/10.1007/s10295-010-0736-3