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Comparison of the spores of Paenibacillus polymyxa prepared at different temperatures

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Abstract

Paenibacillus polymyxa SQR-21, which is antagonistic against Fusarium oxysporum, is used as a biocontrol agent and, when mixed with organic substances for solid fermentation, produces a bioorganic fertilizer. The spores of P. polymyxa prepared at different temperatures were characterized with respect to the dipicolinic acid content, heat resistance, fatty acid composition and germination. Spores prepared at 37°C showed higher heat resistance than those prepared at 25 and 30°C. However, the germination rate was negatively correlated with the sporulation temperature. The maximum germination rate of the spores prepared at 25°C was 1.3-times higher than the spores prepared at 30°C. The sporulation temperature thus affects the resistance and germination properties of P. polymyxa spores. These results are useful for the production of improved bio-organic fertilizer.

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Acknowledgments

This research work was financially supported by Chinese Ministry of Agriculture (201103004), Nature Science Foundation of China (40871126) and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, RZ was supported by Chinese Ministry of Science and Technology (2011BAD11B03-02).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China

    Zhenhua Huo, Nan Zhang, Waseem Raza, Xinqi Huang, Xiaoyu Yong, Yunpeng Liu, Dandan Wang, Shuqing Li, Qirong Shen & Ruifu Zhang

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  1. Zhenhua Huo
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  2. Nan Zhang
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  3. Waseem Raza
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  4. Xinqi Huang
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  6. Yunpeng Liu
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  8. Shuqing Li
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  10. Ruifu Zhang
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Correspondence to Ruifu Zhang.

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Huo, Z., Zhang, N., Raza, W. et al. Comparison of the spores of Paenibacillus polymyxa prepared at different temperatures. Biotechnol Lett 34, 925–933 (2012). https://doi.org/10.1007/s10529-012-0853-3

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  • DOI: https://doi.org/10.1007/s10529-012-0853-3

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