Skip to main content

Heat-Treatment Method for Isolation of Bacillus spp. from Plant Tissues

  • Protocol
  • First Online:
Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Production of spore is an important feature of certain types of bacteria belonging to the members of genera such as Bacillus. Spores are metabolically inactive and in heat-resistant forms. This state occurs due to unfavorable environmental conditions and hinders the vegetative activity of the cell. Spore-forming Bacillus can resist excessive heat due to their chemical composition, therefore, to isolate spore-forming Bacillus species, heat shock and ethanol treatment could be the efficient ways.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Logan NA, de Vos P (2015) Bacillus. In: Bergey’s Manual Trust (ed) Bergey’s manual of systematics of archaea and bacteria. Wiley in association with Bergey’s Manual Trust, Hoboken, pp 1–163. https://doi.org/10.1002/9781118960608.gbm00530

  2. Fritze D (2002) Bacillus identification – traditional approaches. In: Fédération européenne des sociétés de microbiologie (ed) Applications and systematics of bacillus and relatives. Blackwell Publishing, Oxford, pp 8–22

    Google Scholar 

  3. Magotra S, Bhagat N, Ambardar S, Ali T, Hurek BR, Hurek T, Verma PK, Vakhlu J (2021) Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non-traditional areas, and mining of PGP genes from its genome. Sci Rep 11:5454. https://doi.org/10.1038/s41598-021-84585-z

  4. Walker R, Powell AA, Seddon B (1998) Bacillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species. J App Microbiol 84:791–801

    Google Scholar 

  5. Ferreira MEP, Leite LG, Polanczyk RA, Suzuki MT, de Paula Freitas R, da Silva Bueno RN, da Silva RSA (2018) Two methods for isolation of endophytic and edaphic Bacillus spp. from sugarcane fields. Arq Inst Biol 84:1–7

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Kavitha, T.R., Suneetha, C., Sunitha, T.R. (2023). Heat-Treatment Method for Isolation of Bacillus spp. from Plant Tissues. In: Sankaranarayanan, A., Amaresan, N., Dwivedi, M.K. (eds) Endophytic Microbes: Isolation, Identification, and Bioactive Potentials. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2827-0_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2827-0_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2826-3

  • Online ISBN: 978-1-0716-2827-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics