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In vivo evaluation of two types of bioactive scaffold used for tendon–bone interface healing in the reconstruction of anterior cruciate ligament

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Abstract

Fibrin glue combined with bone morphogenetic protein (BMP) and recombined bone xenograft (RBX), were compared to evaluate their effect on the tendon–bone interface healing. The interface of fibrin glue-BMP developed new cartilage but the new bone was thinner whereas the interface of RBX had large areas of chondrocyte-like cells, bone formation and an immature neo-enthesis structure. At 12 weeks, bone mineral density of RBX group (152 ± 52 cm3) and fibrin glue-BMP group (109 ± 13 cm3) was calculated by micro-computed tomography. The ultimate load of fibrin glue-BMP group (60 ± 18 and 51 ± 14 N) and RBX group (65 ± 21 and 57 ± 15 N) was shown by biomechanics at 6 and 12 weeks. RBX thus has an advantage on accelerating tendon–bone interface healing.

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Acknowledgment

The authors thank Dr. Shree R. Singh of National Cancer Institute at National Institutes of Health to help us edit the manuscript.

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Authors and Affiliations

  1. Department of Orthopaedics, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025, People’s Republic of China

    Weibin Zhang & Yiyong Wei

  2. Shanghai Institute of Traumatology and Orthopaedics, Shanghai, 200025, People’s Republic of China

    Weibin Zhang & Yiyong Wei

  3. Department of Human Movement Studies, Xi’an Physical Education University, Xi’an, 710068, People’s Republic of China

    Weimin Pan, Mingjun Zhang & Yiyong Wei

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  1. Weibin Zhang
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  2. Weimin Pan
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  3. Mingjun Zhang
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  4. Yiyong Wei
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Correspondence to Yiyong Wei.

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Zhang, W., Pan, W., Zhang, M. et al. In vivo evaluation of two types of bioactive scaffold used for tendon–bone interface healing in the reconstruction of anterior cruciate ligament. Biotechnol Lett 33, 837–844 (2011). https://doi.org/10.1007/s10529-010-0490-7

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  • DOI: https://doi.org/10.1007/s10529-010-0490-7

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