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Strain-dependent viscoelastic behaviour and rupture force of single chondrocytes and chondrons under compression

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Abstract

The chondron in articular cartilage includes the chondrocyte and its surrounding pericellular matrix (PCM). Single chondrocytes and chondrons were compressed between two parallel surfaces by a micromanipulation technique to investigate their biomechanical properties and to discover the mechanical significance of the PCM. The force imposed on the cells was measured directly during deformation at various compression speeds and deformations up to cell rupture. When the deformation at the end of compression was 50%, relaxation showed that the cells were viscoelastic, but this viscoelasticity was generally insignificant at 30% deformation or lower. When the deformation was 70%, the cells had deformed plastically. Chondrons ruptured at a mean deformation of 85 ± 1%, whilst chondrocytes ruptured at a mean deformation of 78 ± 1%. Chondrons were generally stiffer than chondrocytes and showed less viscoelastic behaviour than chondrocytes. Thus, the PCM significantly influences the mechanical properties of the cells.

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Acknowledgement

We acknowledge the Engineering and Physical Sciences Research Council (EP/C511727/1), UK for sponsoring this work.

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Authors and Affiliations

  1. School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    Bac V. Nguyen, Colin R. Thomas & Zhibing Zhang

  2. Institute for Science & Technology in Medicine, University of Keele, Staffordshire, ST5 5BG, UK

    Qiguang Wang, Nicola J. Kuiper & Alicia J. El Haj

Authors
  1. Bac V. Nguyen
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  2. Qiguang Wang
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  3. Nicola J. Kuiper
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  4. Alicia J. El Haj
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  5. Colin R. Thomas
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  6. Zhibing Zhang
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Correspondence to Zhibing Zhang.

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Nguyen, B.V., Wang, Q., Kuiper, N.J. et al. Strain-dependent viscoelastic behaviour and rupture force of single chondrocytes and chondrons under compression. Biotechnol Lett 31, 803–809 (2009). https://doi.org/10.1007/s10529-009-9939-y

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  • DOI: https://doi.org/10.1007/s10529-009-9939-y

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