Skip to main content
SpringerLink
Log in

A novel micromanipulation technique for measuring the bursting strength of single mammalian cells

  • Biochemical engineering Short contribution
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

Information about the bursting strength of animal cells is essential if the mechanisms of cell damage in bioreactors are to be understood, and if cell mechanical properties are ever to be related to cell structure and physiology. We have developed a novel cell compression technique that makes it possible to directly measure the bursting strength of single mammalian cells, and to infer information about cell mechanical properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Al-Rubeai M, Emery AN (1990) Mechanisms and kinetics of monoclonal antibody synthesis and secretion in synchronous and asynchronous hybridoma cell cultures. J Biotechnol 16:67–86

    Google Scholar 

  • Bird RB, Armstrong RC, Hassager O (1987) Dynamics of polymeric liquids, vol 1, appendix B. Wiley, New York

    Google Scholar 

  • Bleim R (1989) A need for systematic investigations into the material properties of cultured animal cells. Trends Biotechnol 7:197–200

    Google Scholar 

  • Cherry RS, Kwon K (1990) Transient shear stresses on a suspension cell in turbulence. Biotechnol Bioeng 36:563–571

    Google Scholar 

  • Evans EA, Skalak R (1980) Mechanics and thermodynamics of biomembranes. CRC Press, Boca Raton, pp 148–155

    Google Scholar 

  • Hochmuth RM (1987) Properties of red blood cells. In: Skalak R, Chien S (eds) Handbook of bioengineering. McGraw-Hill, New York, pp 12.1–12.17

    Google Scholar 

  • Peterson JF, McIntire LV, Papoutsakis ET (1988) Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentration. J Biotechnol 7:229–246

    Google Scholar 

  • Sato M, Levesque MJ, Nerem RM (1987) An application of the micropipette technique to the measurement of the mechanical properties of cultured bovine aortic endothelial cells. J Biomech Eng 109:27–34

    Google Scholar 

  • Smith CG, Greenfield PF, Randerson DH (1987) A technique for determining the shear sensitivity of mammalian cells in suspension culture. Biotechnol Tech 1:39–44

    Google Scholar 

  • Stathopoulos NA, Hellums JD (1985) Shear stress effects on human embryonic kidney cells in vitro. Biotechnol Bioeng 36:563–571

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK

    Z. Zhang & C. R. Thomas

  2. National Institute for Medical Research, The Ridgeway, NW7 1AA, Mill Hill, London, UK

    M. A. Ferenczi

  3. British Bio-technology Ltd., Watlington Road, OX4 5LY, Oxford, UK

    A. C. Lush

Authors
  1. Z. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Ferenczi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. C. Lush
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. R. Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Offprint requests to: C. R. Thomas

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Z., Ferenczi, M.A., Lush, A.C. et al. A novel micromanipulation technique for measuring the bursting strength of single mammalian cells. Appl Microbiol Biotechnol 36, 208–210 (1991). https://doi.org/10.1007/BF00164421

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00164421

Keywords

Search

Navigation