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A fundamental dual regulatory role of citrate on the biosyntheses of thuringiensin and poly-β-hydroxybutyrate in Bacillus thuringiensis YBT-032

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Abstract

The production of α-ketoglutarate, adenine, thuringiensin production rate and thuringiensin yield on glucose consumed increased by 22%, 36%, 40% and 40%, respectively, in presence of 2 g citrate/l. However, citrate decreased pyruvate production, poly-β-hydroxybutyrate (PHB) production rate and PHB yield by 62%, 31% and 45%, respectively. The activities of pyruvate kinase and glucose-6-phosphate dehydrogenase were 36%–45% lower and 50%–120% higher than those of the control, respectively. The results suggest that citrate regulated the carbon flux to synthesis of adenine present in thuringiensin with a higher efficiency of utilization of glucose by decreasing PHB synthesis.

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Acknowledgement

This study was funded by the Natural Science Foundation of China (No. 30170032). The authors thank Dr. Jeffery K. Tomberli for the valuable discussion on the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China

    Zhi Wang, Shouwen Chen, Ming Sun & Ziniu Yu

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  1. Zhi Wang
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  2. Shouwen Chen
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  3. Ming Sun
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  4. Ziniu Yu
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Correspondence to Ziniu Yu.

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Wang, Z., Chen, S., Sun, M. et al. A fundamental dual regulatory role of citrate on the biosyntheses of thuringiensin and poly-β-hydroxybutyrate in Bacillus thuringiensis YBT-032. Biotechnol Lett 29, 779–784 (2007). https://doi.org/10.1007/s10529-006-9302-5

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  • DOI: https://doi.org/10.1007/s10529-006-9302-5

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