Abstract
The operation of a fed-batch culture is more complicated than that of batch or continuous culture. Thus, an appropriate feeding strategy for fed-batch cultures should be carefully designed. In this study, a simple feeding strategy for fed-batch culture of Bacillus thuringiensis based on “motile intensity” is described. The feeding strategy consisted of two steps: (1) initiating feeding at the peak of motile intensity; (2) terminating feeding at low motile intensity (or non-motility) of the cells. In addition, the motile intensity of B. thuringiensis was used to determine the optimum environmental conditions (pH, temperature, and dissolved oxygen) and optimum medium composition. Using this fed-batch strategy, the production of thuringiensin increased 34% compared with batch culture using the same environmental conditions and medium composition. The proposed strategy for fed-batch culture helps to avoid overfeeding of substrate and facilitates on-line control. A comparison of several alternative strategies for fed-batch culture demonstrated that strategies such as glucose-stat and DO-stat result in a lower productivity than that obtained using the motility intensity method.
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Albertini AM, Caramori T, Crabb WD, Scoffone F, Galizzi A (1991) The fla A locus of Bacillus subtilis is part of a large operon coding for flagellar structures, motility functions, and ATPase-like Polypeptide. J Bacteriol 173:3573–3579
Alexandre G, Greer SE, Zhulin IB (2000) Energy taxis is the dominant behavior in Azospirillum brasilense. J Bacterol 182:6042–6048
Arcas J, Yantorno O, Ertola R (1987) Effect of high concentration of nutrients Bacillus thuringiensis on cultures. Biotechnol Lett 9:105–110
Jong JZ, Hsiun DY, Wu WT (1995) Fed-batch culture of Bacillus thuringiensis for thuringiensin production in a tower type bioreactor. Biotechnol Bioeng 48:207–213
Korber DR, Lawrence JR, Caldwell DE (1994) Effect of motility on surface colonization and reproductive success of Pseudomonas fluorescens in dual-dilution continuous culture and batch culture systems. Appl Environ Microbiol 60:1421–1429
Lee J, Lee SY, Park S, Middelberg APJ (1999) Control of fed-batch fermentations. Biotechiol Adv 17: 29–48
Lecadet MM, Blondel MO, Ribier J (1980) Generalized transduction in Bacillus thuringiensis var Berliner-1715 using bacteriophage-CP-54Ber. J Gen Microbio 121: 203–212
Levinson B, Kasyan KJ, Chiu SS (1990. Identification of β-exotoxin production, plasmids encodingβ-exotoxin and a new exotoxin in by HPLC. J Bacteriol 172: 3172–3179
Martinez A, Torello S, Kolter R (1999) Sliding motility in Mycobacteria. J Bacteriol 181:7331–7338
Mendelson NH, Bourque A, Wilkening K, Anderson KR, Watkins JC (1999) Organized cell swimming motions in Bacillus subtilis colonies: patterns of short-lived whirls and jets. J Bacteriol 181:600–609
Ormonde P, Hörstedt P, O’Toole R, Milton DL (2000) Role of motility in adherence to and invasion of a fish cell line by Vibrio anguillarum. J Bacteriol 182: 2326–2328
Paige MR, Cooper RD (1990) Scale-up of beta exotoxin production in fed batch Bacillus thuringiensis fermentation. Eur Congr Biotechnol 5th Meeting, pp 146–159
Pearson D, Ward OP (1988) Effect of culture conditions on growth and sporulation of Bacillus thuringiensis subsp. israelensis and development of media for production of the protein crystal endotoxin. Biotechnol Lett 10:451–456
Phillips BR, Quinn JA, Goldfine H (1994) Random motility of swimming bacteria: single cells compared to cell populations. AIChE J 2:334–348
Romagnoli S, Armitage JP (1999) Roles of chemosensory pathways in transient changes in swimming speed of Rhodobacter sphaeroldes induced by changes in photosynthetic electron transport. J Bacteriol 181:34–39
Tanigoshi LK, Mayer DF, Babcock HM (1990) Efficacy of the β-exotoxin of Bacillus thuringiensis to Lygus hesperus. J Econ Entomol 83: 2200–2206
Taylor BL, Zhulin LB (1998) In search of higher energy: metabolism-dependent behavior in bacteria. Mol Microbiol 28:683–690
Wu WT, Chen S (2001)An index for determining the culture time of inoculum in cultivation of Bacillus thuringiensis. Biotechnol Lett 23:1201–1204
Wu WT, Chen S, Hong JY (2001) A droplet medium selection for cultivation of Bacillus thuringiensis. Biotechnol Prog 17:778–780
Zouari N, Jaoua S (1999) The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus thuringiensis subsp kurstaki. J Ind Microbiol Biotechnol 23:497–502
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Chen, S., Hong, JY. & Wu, WT. Fed-batch culture of Bacillus thuringiensis based on motile intensity. J IND MICROBIOL BIOTECHNOL 30, 677–681 (2003). https://doi.org/10.1007/s10295-003-0098-1
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DOI: https://doi.org/10.1007/s10295-003-0098-1