Abstract
Protein-coated microcrystals (PCMC), a biocatalyst preparation previously demonstrated to display substantially increased transesterification activity of proteases and lipases in organic solvents when compared to their as received counterparts [Kreiner M, Moore BD, Parker MC (2001) Chem. Commun. 12:1096--1097], was applied to oxidoreductases. Horse liver alcohol dehydrogenase (HLADH), catalase (CAT), soybean peroxidase and horseradish peroxidase were immobilised onto K2SO4 crystals and dehydrated in a 1-step process, resulting in PCMC. These PCMC preparations showed enhanced activity in different organic solvents in most types of reactions tested. The highest activation was observed with HLADH (50-fold as active as enzyme as received) and CAT (25-fold).
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Kreiner, M., Parker, MC. Protein-coated Microcrystals for use in Organic Solvents: Application to Oxidoreductases. Biotechnol Lett 27, 1571–1577 (2005). https://doi.org/10.1007/s10529-005-1800-3
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DOI: https://doi.org/10.1007/s10529-005-1800-3