Abstract
Streptomyces are one of the most prolific sources of bioactive and structurally diverse secondary metabolites for natural product drug discovery. Genome sequencing and bioinformatics analysis revealed that the genomes of Streptomyces harbor a wealth of cryptic secondary metabolite biosynthetic gene clusters that could encode novel compounds. In this work, a genome mining approach was employed to investigate the biosynthetic potential of Streptomyces sp. HP-A2021, isolated from rhizosphere soil of Ginkgo biloba L. The complete genome of HP-A2021 was sequenced and contained the 9,607,552 base pair linear chromosome with a GC content of 71.07%. The annotation results revealed the presence of 8534 CDSs, 76 tRNA genes, and 18 rRNA genes in HP-A2021. The highest dDDH and ANI values based on genome sequences between HP-A2021 and the most closely related type strain, Streptomyces coeruleorubidus JCM 4359, were 64.2% and 92.41%, respectively. In total, 33 secondary metabolite biosynthetic gene clusters with an average length of 105,594 bp were identified, including the putative thiotetroamide, alkylresorcinol, coelichelin, and geosmin. The antibacterial activity assay confirmed that the crude extracts of HP-A2021 showed potent antimicrobial activity against human pathogenic bacteria. Our study demonstrated that Streptomyces sp. HP-A2021 will propose a potential use in biotechnological and novel bioactive secondary metabolite biosynthetic applications.
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Data Availability
The DNA sequences in this study are deposited in the GenBank database, and the accession numbers are provided in the manuscript. Streptomyces sp. HP-A2021 has been deposited in China Center for Type Culture Collection (CCTCC) in Wuhan under the accession number CCTCC AA 2022008.
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Funding
The present study was funded by the Shandong natural resources fund (ZR2022QB146, ZR2019MC036), Heze university doctoral fund projects (XY21BS35), and Shandong key research and development program (2021SFGC1205).
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L-FL performed data curation, formal analysis, investigation, methodology, visualization, writing—original draft; Q-XW, HW, YL, QP, R-HH did data curation and validation; D-HZ, W-DY contributed to supervision and formal analysis; RX gave resources; JW did resources and supervision; ZF was involved in funding acquisition and project administration; S-YH done funding acquisition, project administration, investigation, supervision, writing—review & editing; all authors reviewed the manuscript.
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Li, LF., Wu, QX., Wu, H. et al. Complete Genome Sequence of Streptomyces sp. HP-A2021, a Promising Bacterium for Natural Product Discovery. Biochem Genet 61, 2042–2055 (2023). https://doi.org/10.1007/s10528-023-10350-8
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DOI: https://doi.org/10.1007/s10528-023-10350-8