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Circ_0001821 Potentiates Cell Growth, Metastasis, and Stemness in Colorectal Cancer by Regulating miR-339-3p/CST1

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Abstract

Dysregulation of circRNAs is associated with human cancer progression, but its expression pattern and function in cancer are not fully understood. The aim of our study is to investigate the effects of circ_0001821 on colorectal cancer (CRC) proliferation, migration, invasion, and stemness. The expression level of circ_0001821 in CRC tissues and cells was detected by quantitative real-time PCR. The effects of circ_0001821 silencing on the proliferation, migration, invasion, and stemness of CRC cells were examined by cell counting kit-8, 5-Ethynyl-2′-deoxyuridine, transwell, sphere formation, and western blot assays. Bioinformatics and dual-luciferase reporter gene assay were used to verify the relationship between circ_0001821 and miR-339-3p, miR-339-3p and CST1 in CRC cells. Circ_0001821 was highly expressed in CRC tissues and cells. Circ_0001821 silencing inhibited the proliferation, migration, invasion, and stemness of CRC cells, and transfection of miR-339-3p inhibitor partly attenuated this effect. In addition, circ_0001821 can bind miR-339-3p to regulate CST1 expression. Circ_0001821 silencing also curbed tumor growth in vivo. Circ_0001821 promoted CRC cell proliferation, migration, invasion, and stemness by regulating the miR-339-3p/CST1 axis.

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References

  • Buccafusca G et al (2019) Early colorectal cancer: diagnosis, treatment and survivorship care. Crit Rev Oncol Hematol 136:20–30

    Article  PubMed  Google Scholar 

  • Chatila R et al (2021) Epidemiology and survival of colorectal cancer in Lebanon: a sub-national retrospective analysis. Cancer Control 28:10732748211041220

    Article  PubMed  PubMed Central  Google Scholar 

  • Chen C et al (2020) The circular RNA 001971/miR-29c-3p axis modulates colorectal cancer growth, metastasis, and angiogenesis through VEGFA. J Exp Clin Cancer Res 39(1):91

    Article  PubMed  PubMed Central  Google Scholar 

  • Chen S et al (2021) CST1 promoted gastric cancer migration and invasion through activating Wnt pathway. Cancer Manag Res 13:1901–1907

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dai DN et al (2017) Elevated expression of CST1 promotes breast cancer progression and predicts a poor prognosis. J Mol Med (berl) 95(8):873–886

    Article  CAS  PubMed  Google Scholar 

  • Flemming A (2019) The enigma of circular RNA. Nat Rev Immunol 19(6):351

    Article  CAS  PubMed  Google Scholar 

  • Hansen TB et al (2013) Natural RNA circles function as efficient microRNA sponges. Nature 495(7441):384–388

    Article  CAS  PubMed  Google Scholar 

  • Hao S et al (2019) Emerging roles of circular RNAs in colorectal cancer. Onco Targets Ther 12:4765–4777

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang J et al (2018) Let7d inhibits colorectal cancer cell proliferation through the CST1/p65 pathway. Int J Oncol 53(2):781–790

    CAS  PubMed  Google Scholar 

  • Jin YD et al (2019) Hsa_circ_0005075 predicts a poor prognosis and acts as an oncogene in colorectal cancer via activating Wnt/beta-catenin pathway. Eur Rev Med Pharmacol Sci 23(8):3311–3319

    PubMed  Google Scholar 

  • Keum N, Giovannucci E (2019) Global burden of colorectal cancer: emerging trends, risk factors and prevention strategies. Nat Rev Gastroenterol Hepatol 16(12):713–732

    Article  PubMed  Google Scholar 

  • Kong S et al (2019) Identification of hsa_circ_0001821 as a novel diagnostic biomarker in gastric cancer via comprehensive circular RNA profiling. Front Genet 10:878

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Y et al (2015) Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis. Cell Res 25(8):981–984

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Y et al (2019) A novel circFMN2 promotes tumor proliferation in CRC by regulating the miR-1182/hTERT signaling pathways. Clin Sci (lond) 133(24):2463–2479

    Article  CAS  PubMed  Google Scholar 

  • Li G et al (2021) CircRNA hsa_circ_0014130 function as a miR-132–3p sponge for playing oncogenic roles in bladder cancer via upregulating KCNJ12 expression. Cell Biol Toxicol 38:1079–1096

    Article  PubMed  Google Scholar 

  • Li S et al (2021) CircDOCK1 promotes the tumorigenesis and cisplatin resistance of osteogenic sarcoma via the miR-339-3p/IGF1R axis. Mol Cancer 20(1):161

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li C et al (2022) High expression of circ_0001821 promoted colorectal cancer progression through miR-600/ISOC1 axis. Biochem Genet 2022:1–18

    Google Scholar 

  • Liang R, Tang Y (2020) LINC00467 knockdown repressed cell proliferation but stimulated cell apoptosis in glioblastoma via miR-339-3p/IP6K2 axis. Cancer Biomark 28(2):169–180

    Article  CAS  PubMed  Google Scholar 

  • Liu L, Zhang F, Li J (2021a) CircRNA circ_0001821 predicts an unfavorable prognosis and promotes the proliferation of multiple myeloma. Hematology 26(1):716–723

    Article  CAS  PubMed  Google Scholar 

  • Liu Y et al (2021b) Circ_0001821 knockdown suppresses growth, metastasis, and TAX resistance of non-small-cell lung cancer cells by regulating the miR-526b-5p/GRK5 axis. Pharmacol Res Perspect 9(4):e00812

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Panda AC (2018) Circular RNAs Act as miRNA sponges. Adv Exp Med Biol 1087:67–79

    Article  CAS  PubMed  Google Scholar 

  • Rupaimoole R, Slack FJ (2017) MicroRNA therapeutics: towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov 16(3):203–222

    Article  CAS  PubMed  Google Scholar 

  • Siegel RL et al (2017) Colorectal cancer statistics, 2017. CA Cancer J Clin 67(3):177–193

    Article  PubMed  Google Scholar 

  • Simon K (2016) Colorectal cancer development and advances in screening. Clin Interv Aging 11:967–976

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sung H et al (2021) Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 71(3):209–249

    Article  PubMed  Google Scholar 

  • Vo JN et al (2019) The landscape of circular RNA in cancer. Cell 176(4):869-881 e13

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Weber CE et al (2016) miR-339-3p is a tumor suppressor in melanoma. Cancer Res 76(12):3562–3571

    Article  CAS  PubMed  Google Scholar 

  • Xue M et al (2020) Circular RNA circ-LDLRAD3 serves as an oncogene to promote non-small cell lung cancer progression by upregulating SLC1A5 through sponging miR-137. RNA Biol 17(12):1811–1822

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang Z, Deng X (2022) circ_0001821 contributes to the development of cutaneous squamous cell carcinoma by regulating MicroRNA-148a-3p/EGFR axis and activating phosphatidylinositol 3-kinase/Akt pathway. Mol Cell Biol 42(3):e0008921

    Article  PubMed  Google Scholar 

  • Zhao ZJ, Shen J (2017) Circular RNA participates in the carcinogenesis and the malignant behavior of cancer. RNA Biol 14(5):514–521

    Article  PubMed  Google Scholar 

  • Zhou C, Lu Y, Li X (2015) miR-339-3p inhibits proliferation and metastasis of colorectal cancer. Oncol Lett 10(5):2842–2848

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhou X et al (2021) Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer. J Exp Clin Cancer Res 40(1):124

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of General Surgery, Suzhou Hospital of Anhui Medical University, No. 22, Nanyuan District, Suzhou, 234000, Anhui, China

    Changqing Liu & Tianming Mei

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  1. Changqing Liu
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  2. Tianming Mei
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CL designed and performed the research; CL and TM analyzed the data; CL wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Tianming Mei.

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This study was permitted by the Ethics Committee of Suzhou Hospital of Anhui Medical University.

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Liu, C., Mei, T. Circ_0001821 Potentiates Cell Growth, Metastasis, and Stemness in Colorectal Cancer by Regulating miR-339-3p/CST1. Biochem Genet 61, 1451–1469 (2023). https://doi.org/10.1007/s10528-022-10329-x

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  • DOI: https://doi.org/10.1007/s10528-022-10329-x

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