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CircPACRGL promoted cell proliferation, migration and invasion as well as inhibited cell apoptosis in colorectal cancer via regulation of the miR-330-3p/CNBP axis

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Abstract

CircRNAs are a member of noncoding RNAs and have been verified to play an important regulatory role in cancers. In CRC, the regulatory mechanisms of various circRNAs have not been elucidated. The expression of circPACRGL and miR-330-3p was detected with qRT-PCR. The protein expression of CDK4, MMP-9, Bcl-2, Bax, cellular nucleic acid-binding protein (CNBP) and β-actin was measured with western blot. Cell proliferation was analyzed using MTT assay, colony formation assay, and EDU assay. Cell apoptosis was detected using flow cytometry. Cell migration and invasion were measured with wound healing and transwell invasion assay. Luciferase reporter assay and RIP assay was used to determine the relationship of among miR-330-3p, circPACRGL and CNBP in CRC cells. In this study, we found that circPACRGL and CNBP expressed high and miR-330-3p expressed low in CRC tissues and cells. Functional experiments showed that inhibition of circPACRGL reduced cell proliferation, migration and invasion in CRC. In addition, knockdown of circPACRGL contributed to cell apoptosis in CRC. Dual-luciferase report assay determined that circPACRGL was a miR-330-3p sponge molecular and CNBP was a target of miR-330-3p. Reversed experiments showed that the effects of sh-circPACRGL transfection on CRC cells were rescued by up-regulating CNBP expression. In this study, we for the first time found a novel regulatory network of circPACRGL in CRC. The results manifested that circPACRGL affected tumor growth by targeting miR-330-3p/CNBP axis in CRC, highlighting the potential of circPACRGL as a therapeutic target for colorectal cancer.

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Acknowledgements

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Funding

This work was supported by Funded project of “Double first-class” discipline (Traditional Chinese Medicine) construction in Jiangxi Province [NO.JXSYLXK-ZHY1054]; Science and Technology Research Project of Education Department of Jiangxi Province [No.201210, 200143]; Scientific planning and Planning Fund project of Jiangxi Health Commission [NO.20195637, 20165401]; Science and Technology Project of Jiangxi Administration of Traditional Chinese Medicine [NO.2019A106]; 2019 University-level Innovation and Entrepreneurship Training Plan for College Students of Jiangxi University of Chinese Medicine [NO.201910412045]; Csco-Hengrui Cancer Research Fund (Y-HR2017-128).

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    Authors and Affiliations

    1. College of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China

      Haiyun Liu, Yongqing Fang, Qianxia Lin, Wenchun Zhang & Xiaomin Wang

    2. Department of Radiation Oncology, Jiangxi Cancer Hospital, NO.519 East Beijing Road Street, Nanchang, Jiangxi, China

      Zhijian He

    3. School of Inter-Cultural Studies, Jiangxi Normal University, Nanchang, Jiangxi, China

      Yuan Hu

    4. Research Center for Differentiation and Development of Traditional Chinese Medicine (TCM) Basic Theory, Jiangxi University of Chinese Medicine, No.1688 Meiling Avenue, Nanchang, 330004, Jiangxi, China

      Guoliang Xu

    5. Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, No.17 Yongwaizheng Avenue, Donghu District, Nanchang, 330006, Jiangxi, China

      Benchao Hou

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    All authors have been involved in the management of the patient and in the conception of the manuscript. The specific authors’ contribution is as follows. Haiyun Liu and Zhijian He: writing the drafting of the manuscript. Qianxia Lin, Wenchun Zhang and Xiaomin Wang: data collection. Yulong Ji, Yongqing Fang and Yuan Hu: analysis and interpretation. Xinrong Hu: software, reviewing and editing.

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    Liu, H., Fang, Y., Hou, B. et al. CircPACRGL promoted cell proliferation, migration and invasion as well as inhibited cell apoptosis in colorectal cancer via regulation of the miR-330-3p/CNBP axis. Mol Cell Biochem 478, 1633–1644 (2023). https://doi.org/10.1007/s11010-022-04543-9

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