Abstract
To verify the quality of triploid varieties of Camellia tea species at the secondary metabolite level, we tested caffeine and catechin profiles of 97 F1 segregating progenies in two breeding populations with a common tetraploid parent and diploid parents of two geographic and varietal origins. Catechin and caffeine levels of the triploid progenies were quantified and compared against their diploid parent. Some of the progenies showed better performance than their diploid parent. Most of the progenies of the diploid C. sinensis × tetraploid cross showed heterosis for caffeine and EGCG. Progenies of the C. assamica subsp. lasiocalyx × tetraploid cross showed heterosis for +C, EC, EGC, and TC. The genomic contributions of the diploid parent seem to be the main factor in the variation between the two populations. Our studies showed quantitative enhancement of some of the quality-related parameters in tea, providing a platform to refocus on this classical breeding approach for developing quality cultivars in tea.
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References
Balasaravanan T, Pius PK, Kumar RR, Muraleedharan N, Shasany AK (2003) Genetic diversity among south Indian tea germplasm (Camellia sinensis, C. assamica and C. assamica spp. lasiocalyx) using AFLP markers. Plant Sci 165:365–372
Bento M, Pereira HS, Rocheta M, Gustafson P, Viegas W, Silva M (2008) Polyploidization as a retraction force in plant genome evolution: sequence rearrangements in Triticale. PLoS One 3(1):e1402
Bezbaruah HP (1968) An evaluation of preparatory procedures for leaf-tip chromosomes spreads of the tea plant (Camellia sinensis). Biotech Histochem 43(5):279–282
Borah A (2010, December 26) Climate change leaves Assam tea growers in hot water; Rising temperatures reducing yields and altering distinctive flavour of India's most popular drink. The Guardian. www.guardian.co.uk/environment/2010/dec/26/climate-change-assam-tea. Accessed August 2012
Caffin NA, D’Arcy B, Yao L, Rintoul G (2004) Developing an index of quality for Australian tea. Rural Industries Research and Development Corp, Kingston
Chen M (2002) Tea and health, an overview. In: Zhen Y, Chen Z, Cheng S, Chen M (eds) Tea: bioactivity and therapeutic potential. Taylor and Francis, London, pp 1–16
Chen L, Zhou ZX (2005) Variations of main quality components of tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in the China national germplasm tea repository. Plant Foods Hum Nutr 60(1):31–35
Collier PD, Mallows R, Thomas PE (1972) Interactions between theaflavins, flavanols and caffeine. Phytochemistry 11:867
Crouch JH, Crouch HK, Tenkouano A, Ortiz R (1999) VNTR-based diversity analysis of 2× and 4× full-sib Musa hybrids. Electron J Biotechnol 2(3):99–108
Das SC, Das S, Hazarika M (2012) Breeding of the tea plant (Camellia sinensis) in India. In: Chen L, Apostolides Z, Chen Z-M (eds) Global tea breeding: achievements, challenges and perspectives. Springer and Zhejiang University Press, Hangzhou
Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87:81–89
Hegarty MJ, Hiscock SJ (2008) Genomic clues to the evolutionary success of polyploid plants. Curr Biol 18(10):R435–R444
Henning SM, Niu Y, Lee NH, Thames GD, Minutti RR, Wang H, Go VLW, Heber D (2004) Bioavailability and antioxidant activity of tea flavanols after consumption of green tea, black tea, or a green tea extract supplement. Am J Clin Nutr 80(6):1558–1564
International Organization for Standardization (2005) Determination of substances characteristic of green and black tea, 2: content of catechins in green tea, method using high-performance liquid chromatography. ISO 14502-2 (Geneva)
Kamunya SM, Wachira FN, Pathak RS, Muoki RC, Wanyoko JK, Ronno WK, Sharma RK (2009) Quantitative genetic parameters in tea (Camellia sinensis (L.) O. Kuntze), I: combining abilities for yield, drought tolerance and quality traits. Afr J Plant Sci 3(5):93–101
Karp A, Edwards KJ (1998) DNA markers: a global overview. In: Anolles GC, Gresshoff PM (eds) DNA markers: protocols, applications and overviews. Wiley-Liss, New York
Kim YS, Hahn EJ, Hosakatte NM, Paek KY (2004) Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng. J Plant Biol 47(4):356–360
Liu Z, Adams KL (2007) Expression partitioning between genes duplicated by polyploidy under abiotic stress and during organ development. Curr Biol 17:1669–1674
Liu SL, Adams KL (2010) Dramatic change in function and expression pattern of a gene duplicated by polyploidy created a paternal effect gene in the Brassicaceae. Mol Biol Evol 27(12):2817–2828
Mandal AKA, Babu S, Senthil Kumar RS (2011) Tea. In: Singh RJ (ed) Genetic resources, chromosome engineering, and crop improvement: medicinal plants, Chapter 18, vol 6. CRC Press, Boca Raton
Mártonfiová L, Majeský U, Mártonfi P (2007) Polyploid progeny from crosses between diploid sexuals and tetraploid apomictic pollen donors in Taraxacum sect. Ruderalia. Acta Biol Crac Ser Bot 49(1):47–54
Moreno LS, Ordoñez SA, Dela Cruz IA, Redoña ED (2000) Relationship of parental genetic diversity with heterosis in two-line and three-line Philippine rice hybrids. In: Advances in rice genetics (Los Baños, Laguna, Philippines, 22-27 October), pp 12–15
Ranney TG (2006) Polyploidy: from evolution to new plant development. Comb Proc Intern Plant Propagators’ Soc 46:137–142
Roberts EAH, Wight W, Wood DJ (1958) Paper chromatography as an aid to the taxonomy of the camellias. New Phytol 57:211–225
Sabhapondit S, Bhuyan LP, Goswami BC, Hazarika M (2011) Seasonal and cultivar variation of catechin in north east Indian tea. Sci Cult 77(9–10):406–411
Sabhapondit S, Karak T, Bhuyan LP, Goswami BC, Hazarika M (2012) Diversity of catechin in northeast Indian tea cultivars. Sci World J. doi:10.1100/2012/485193
Sharma RK, Negi MS, Sharma S, Bhardwaj P, Kumar R, Bhattachrya E, Tripathi SB, Vijayan D, Baruah AR, Das SC, Bera B, Rajkumar R, Thomas J, Sud RK, Muraleedharan N, Hazarika M, Lakshmikumaran M, Raina SN, Ahuja PS (2010) AFLP-based genetic diversity assessment of commercially important tea germplasm in India. Biochem Genet 48:549–564
Singh ID (1980) Non-conventional approaches to the breeding of tea in north-east India. Two and A Bud 27:3–6
Singh ID (1992) A decade of tea breeding in north east India. In: Proceedings of the 31st Tocklai Conference, Tocklai Experimental Station, Jorhat
Singh P, Singh S, Mishra SP, Bhatia SK (2010) Molecular characterization of genetic diversity in Jatropha curcas L. Genes Genom Genomics 4:1–8
Soltis PS, Soltis DE (2000) The role of genetic and genomic attributes in the success of polyploids. Proc Natl Acad Sci USA 97(13):7051–7057
Wang H, Provan GJ, Helliwell K (2000) Tea flavonoids: their functions, utilisation and analysis. Trends Food Sci Technol 11:152–160
Yang CS, Chung JY, Yang G, Chhabra SK, Lee MJ (2000) Tea and tea polyphenols in cancer prevention. J Nutr 130:472S–478S
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The authors are thankful to all the members of the Department of Biotechnology, Tocklai Experimental Station, for their constant support. We are grateful for the funding provided by Department of Biotechnology, Government of India.
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Das, S.K., Sabhapondit, S., Ahmed, G. et al. Biochemical Evaluation of Triploid Progenies of Diploid × Tetraploid Breeding Populations of Camellia for Genotypes Rich in Catechin and Caffeine. Biochem Genet 51, 358–376 (2013). https://doi.org/10.1007/s10528-013-9569-x
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DOI: https://doi.org/10.1007/s10528-013-9569-x