Abstract
Adventitious roots ofPanax ginseng C.A. Meyer (a natural tetraploid) were treated with 50 or 100 mg L-1 colchicine for 12, 24,36, 48, or 60 h to induce polyploid (octoploid) roots. The largest number of octoploid roots was obtained with a 100 mg L-1 colchicine treatment over 60 h. To verify that ginsenoside was being accumulated in the developing tissues, the tetraploid (control) and octoploid roots were cultured for 40 d in Murashige and Skoog media that lacked NH4NO3 but was supplemented with 2 mg L-1 naphthaleneacetic acid and 50 g L-1 sucrose. Levels of fresh and dry biomass were greater in the octoploid roots. Although total ginsenoside and Rb-group ginsenoside contents were less in the octoploid roots than in the tetraploids, the former had a higher amount of Rg-group ginsenosides (especially Rg1). These results demonstrate the benefit that polyploid adventitious roots provide in enhancing the production of secondary metabolites in ginseng.
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Literature Cited
Bulgakov VP, Lauve LS, Tchernoded GK, Khodakovskaya MV, Zhuravlev YN (2000) Chromosome variation in ginseng cells transformed with thero/C plant oncogene. Russ J Genet 36:150–156
Chen XC, Zhu YG, Zhu LA, Huang C, Chen Y, Chen LM, Fang F, Zhou YC, Zhao CH (2003) Ginsenoside Rg1 attenuates dopamine-induced apoptosis in PC12 cells by suppressing oxidative stress. Eur J Pharmacol 473:1–7
De Jesus-Gonzalez L, Weathers PJ (2003) TetraploidArtemisia annua hairy roots produce more artemisinin than diploids. Plant Cell Rep 21:809–813
Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: A review. Euphytica 87:81–89
Gao SL, Zhu DN, Cai ZH, Xu DR (1996) Autotetraploid plants from colchicine-treated bud culture ofSalvia miltiorrhiza Bge. Plant Cell Tiss Org Cult 47:73–77
Griesbach RJ, Kamo KK (1996) The effect of induced polyploidy on the flavonoids ofPetunia Mitchell. Phytochem 42:361–363
Grushvitskii IV (1961) Zhenshen: Voprosy Biologii (Ginseng: Problems in Biology), Leningrad
Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panaxginseng C. A. Meyer. In Vitro Cell Dev Biol Plant 39:245–249
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497
Palazon J, Cusido RM, Bonfill M, Mallol A, Moyano E, Morales C, Pinol MT (2003) Elicitation of differentPanax ginseng transformed root phenotypes for an improved ginsenoside production. Plant Physiol Biochem 41:1019–1025
Shin YH, Kim SC, Han JW, Oh JY, Jeong OM, Shin DH, Nah SY (1997) Ginseng protopanaxatriol saponins but not protopanaxadiol saponins inhibit spontaneous motility of intestine. Koryo Insam Hakheochi 21:35–38
Svehlikova V, Repcak M (2000) Variation of apigenin quantity in diploid and tetraploidChamomilla rectita (L.) Rauschert. Plant Biol 2:403–407
Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture ofPanax ginseng C.A. Meyer. Biochem Eng J 11:211–215
Zheng GZ, Yang CR (1994) Sanchi (Panax notoginseng) Biology and Application. Science Press, Beijing
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Kim, YS., Hahn, EJ., Murthy, H.N. et al. Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng. J. Plant Biol. 47, 356–360 (2004). https://doi.org/10.1007/BF03030551
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DOI: https://doi.org/10.1007/BF03030551