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Evaluation of Genetic Variation in the Clown Knifefish, Chitala chitala, Using Allozymes, RAPD, and Microsatellites

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Abstract

Twenty-seven enzyme systems, six random amplified polymorphic DNA (RAPD) primers, and two microsatellite loci were tested to determine intraspecific divergence in the natural population of the endangered Indian featherback fish, Chitala chitala, for the first time. The 262 samples of C. chitala were collected from six riverine locations in India: the Satluj, Ganga (Ghagra, Bhagirathi, and Brahmaputra), Mahanadi, and Narmada river systems. The analysis revealed population subdivisions, with an FST value from 0.1235 (95% confidence 0.0868–0.1621) for RAPD and a combined FST of 0.0344 (95% confidence 0.0340–0.0350) for microsatellite loci. An analysis of 38 allozyme loci did not reveal any polymorphism in the samples from any of the riverine localities; a possible explanation for this could be that the ancestors of Chitala could have faced a population reduction in prehistoric periods, as low allozyme variation is also reported for other species of Chitala from south Asia.

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Acknowledgments

Financial support received from the NATP-ICAR (subproject MM-18) is thankfully acknowledged. Excellent technical assistance provided by Mr. R. S. Sah, Mr. Akhilesh Mishra, Mr. Rajesh Kumar, and Mr. Sree Ram is also duly acknowledged.

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Authors and Affiliations

  1. National Bureau of Fish Genetic Resources (ICAR), Canal Ring Road, P.O. Dilkusha, Lucknow, 226002, UP, India

    Anup Mandal, Kuldeep Kumar Lal, Vindhya Mohindra, Rajeev Kumar Singh, Peyush Punia & Wazir Singh Lakra

  2. Department of Environmental Sciences, APS University, Rewa, MP, India

    U. K. Chauhan

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  1. Anup Mandal
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  2. Kuldeep Kumar Lal
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  3. Vindhya Mohindra
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Correspondence to Kuldeep Kumar Lal.

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Mandal, A., Lal, K.K., Mohindra, V. et al. Evaluation of Genetic Variation in the Clown Knifefish, Chitala chitala, Using Allozymes, RAPD, and Microsatellites. Biochem Genet 47, 216–234 (2009). https://doi.org/10.1007/s10528-008-9219-x

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