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Thermolabile Variant, PHOX-S, of Prophenol Oxidase in Drosophila melanogaster

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Abstract

The PhoxS strain of Drosophila melanogaster is an electrophoretically slow variant found in a wild population at Victoria, Australia. Prophenol oxidase isoform A1 from PHOX-S was purified and characterized biochemically and genetically. The purified fraction of A1 from PHOX-S showed a homodimer with a molecular weight of the subunit of approximately 77 kDa. The PhoxS strain was temperature sensitive in vivo in culture, and the purified protein was thermolabile in vitro. By the deletion mapping method, the PhoxS locus was cytologically estimated to be at the location 55-A on the right arm of the second chromosome and 79.6 genetically. These data show that PHOX-S is an electrophoretic variant of MOX and that PHOX-S is the first thermolabile protein found in invertebrate prophenol oxidase.

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Authors and Affiliations

  1. Biological Laboratory, Faculty of Science, Okayama University of Science, Okayama, 700-0005, Japan

    Nobuhiko Asada, Satoko Kitamura & Mitsuo Tanaka

  2. Chemical Laboratory, Faculty of Science, Okayama University of Science, Okayama, 700-0005, Japan

    Shigeo Yamazaki

  3. Niimi College, Niimi, 718-8585, Japan

    Masayoshi Namba

Authors
  1. Nobuhiko Asada
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  2. Satoko Kitamura
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  3. Mitsuo Tanaka
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  4. Shigeo Yamazaki
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  5. Masayoshi Namba
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Correspondence to Nobuhiko Asada.

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Asada, N., Kitamura, S., Tanaka, M. et al. Thermolabile Variant, PHOX-S, of Prophenol Oxidase in Drosophila melanogaster. Biochem Genet 43, 549–560 (2005). https://doi.org/10.1007/s10528-005-8171-2

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  • DOI: https://doi.org/10.1007/s10528-005-8171-2

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