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Impact of temperature on the survival and the biocontrol efficacy of Lysobacter capsici AZ78 against Phytophthora infestans

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Abstract

Temperature can strongly influence the biocontrol efficacy of Lysobacter strains when used against phytopathogens. We investigated the influence of temperature on physiological characteristics of Lysobacter capsici AZ78 in a biocontrol situation. L. capsici AZ78 effectively colonized tomato leaves at 15 and 25 °C while exposure to 35 and 37 °C was highly detrimental. Accordingly, Phytophthora infestans attacks on tomato leaves were controlled when L. capsici AZ78 was pre-exposed to 15 and 25 °C for 24 h. Conversely, exposure to 35 °C drastically reduced the biocontrol efficacy of L. capsici AZ78 probably by impairing anti-oomycete and proteolytic activity. Furthermore, the exposure to 15 °C made the L. capsici AZ78 cells more resistant to UV light and copper ions compared to 25 and 35 °C. These findings give an indication on the best temperature range for the future use of biofungicides based on L. capsici AZ78 cells.

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Acknowledgments

The authors wish to thank Denise Ress for her technical assistance and Dr. Valerio Mazzoni for discussion on statistical analysis. This research was supported by the EU project CO-FREE (theme KBBE.2011.1.2-06, Grant agreement number 289497).

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Authors and Affiliations

  1. Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all’Adige, TN, Italy

    Gerardo Puopolo, Maria Cristina Palmieri, Oscar Giovannini & Ilaria Pertot

Authors
  1. Gerardo Puopolo
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  2. Maria Cristina Palmieri
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  3. Oscar Giovannini
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  4. Ilaria Pertot
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Corresponding author

Correspondence to Gerardo Puopolo.

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Handling Editor: Monica Hofte.

Electronic supplementary material

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10526_2015_9672_MOESM1_ESM.docx

Fig. S1 Effect of temperature on in vitro growth of Lysobacter capsici AZ78 in liquid medium. AZ78 cell growth was monitored over 144 h by measuring optical density at 600 nm (OD600nm) spectrophotometrically. Points indicate mean ± SE (n = 6). Supplementary material 1 (DOCX 174 kb)

10526_2015_9672_MOESM2_ESM.docx

Table S1 Presence of viable Lysobacter capsici AZ78 cells on the leaves of tomato plants exposed to different temperatures. Colonisation of tomato leaves by AZ78 was monitored one day (T1) and eight days (T2) after its application. AZ78 populations are expressed as log10 CFU g−1 of leaf. Values (mean values ± SE, n = 12) with different letters are significantly different between treatments according to Tukey’s test (P < 0.05). Supplementary material 2 (DOCX 15 kb)

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Puopolo, G., Palmieri, M.C., Giovannini, O. et al. Impact of temperature on the survival and the biocontrol efficacy of Lysobacter capsici AZ78 against Phytophthora infestans . BioControl 60, 681–689 (2015). https://doi.org/10.1007/s10526-015-9672-5

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  • DOI: https://doi.org/10.1007/s10526-015-9672-5

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