Abstract
Seed lots of winter wheat and rye, naturally infested with Microdochium nivale and Fusarium spp., were treated with an isolate of Pseudomonas, which was recovered from roots of Brassica napus. Seeds were treated with bacterial fermentate and dried before sowing or they were directly sprayed in the furrow-opener at the moment of sowing. Besides field experiments, parallel climate chamber bioassays were performed to assess the effect of bacterial treatment on snow mould caused by seed-borne M. nivale and Fusarium spp. The biocontrol effect was assessed by plant density counts and by measuring yield. Significant biocontrol activity, measured by plant density counts, was detected both in field and climate chamber experiments sown with wheat. Biocontrol effect after spray application at sowing was less pronounced, although a slight increase in plant density was observed. The cell concentration required to obtain adequate biocontrol effect was 109 CFU per ml for the dose used. The bacterial isolate was identified by 16S rDNA sequencing and biochemical tests as a Pseudomonas brassicacearum strain.
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Acknowledgements
This work was conducted within the research program DOM (Domestication of non-conventional Microorgansims) and funded by MISTRA (The foundation for strategic environmental research (http://www.mistra.org)). We thank Ms. Annika Gustafsson for excellent technical assistance, Dr. Lennart Johnsson for sharing his vast experience about field experiments in cereals, Mr. Carl Westberg and Mr. Per Vretblad for engineering the spray-equipment into the experimental sowing machine, Mr. Per Widén for providing naturally infected seed lots, and Dr. Olga Vinnere for support with PAUP-analysis.
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Levenfors, J.P., Eberhard, T.H., Levenfors, J.J. et al. Biological control of snow mould (Microdochium nivale) in winter cereals by Pseudomonas brassicacearum, MA250. BioControl 53, 651–665 (2008). https://doi.org/10.1007/s10526-007-9102-4
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DOI: https://doi.org/10.1007/s10526-007-9102-4