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Combined Application of the Biological Product LS213 with Bacillus, Pseudomonas or Chryseobacterium for Growth Promotion and Biological Control of Soil-Borne Diseases in Pepper and Tomato

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Abstract

Recent works suggest that the combination of several PGPRs could be more effective than individual strains as a horticultural product. LS213 is a product formed by a combination of two PGPRs, Bacillus subtilis strain GB03 (a growth-promoting agent), B. amyloliquefaciens strain IN937a (an inducer of systemic resistance) and chitosan. The aim of this work is to establish if the combination of three PGPR, B. licheniformis CECT 5106, Pseudomonas fluorescens CECT 5398 and Chryseobacterium balustinum CECT 5399 with LS213 would have a synergistic effect on growth promotion and biocontrol on tomato and pepper against Fusarium wilt and Rhizoctonia damping off. When individual rhizobacterium and the LS213 were put together, the biometric parameters were higher than with individual rhizobacterium both in tomato and pepper, revealing a synergistic effect on growth promotion, being the most effective combination that of B. licheniformis and LS213. When P. fluorescens CECT 5398 was applied alone, it gave good results, which could be due to the production of siderophores by this strain. Biocontrol results also indicate that those treatments that combined LS213 and each of the bacteria (Treatments: T7 and T8) gave significantly higher percentages of healthy plants for both tomato (T7: 65%) and pepper (T7: 75% and T8: 70%) than the LS213 alone (45% of healthy plants for tomato and 60% for pepper) three weeks after pathogen attack. The effects in pepper were more marked than in tomato. The best treatment in biocontrol was the combination of P. fluorescens and LS213. In summary, the combination of microorganisms gives better results probably due to the different mechanisms used.

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Abbreviations

CECT:

Colección Española de Cultivos Tipo. Spanish Type Cultures Collection

PGPR:

Plant Growth Promotion Rhizobacteria

ISR:

Induced systemic resistance

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Authors and Affiliations

  1. Departamento de Ciencias Ambientales y Recursos Naturales, Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Ctra. Boadilla Km 5,3, 28668, Boadilla del Monte, Madrid, Spain

    J. Domenech, B. Ramos & J. Gutierrez-Mañero

  2. Department of Entomology and Plant Pathology, Auburn University, 303 Life Sciences Building, Auburn, Alabama, 36849, USA

    M. S. Reddy & J. W. Kloepper

Authors
  1. J. Domenech
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  2. M. S. Reddy
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  3. J. W. Kloepper
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  4. B. Ramos
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  5. J. Gutierrez-Mañero
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Correspondence to J. Domenech.

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Domenech, J., Reddy, M.S., Kloepper, J.W. et al. Combined Application of the Biological Product LS213 with Bacillus, Pseudomonas or Chryseobacterium for Growth Promotion and Biological Control of Soil-Borne Diseases in Pepper and Tomato. Biocontrol 51, 245–258 (2006). https://doi.org/10.1007/s10526-005-2940-z

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  • DOI: https://doi.org/10.1007/s10526-005-2940-z

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