Abstract
Somatotropin (GH) and insulin (INS) low dose, long-term brain rejuvenation effect was studied in the cerebral cortex using NE, EPI, DA and 5-HT receptor subtypes of young (group I—treatment started 4 weeks continued to 16 weeks) and old rats (group II—treatment started 60 weeks continued to 90 weeks). GH and INS treatment showed significant decrease in NE and EPI content in cerebral cortex of both young and old rats. α2A-adrenergic receptors showed decreased expression whereas β2-adrenergic receptors showed enhanced expression with age. GH and INS treatment significantly increased α2A-adrenergic receptor protein in group I rats whereas INS treatment could increase β2-adrenergic receptor protein expression in group II rats. DA and 5-HT content decrease with age. GH and INS administration showed increase in DA and 5-HT content in the brain regions-corpus striatum and brainstem of both young and old rats. Also, DA D2 and 5-HT2C receptor gene expression were increased significantly by GH and INS treatment in both young and old rats. In conclusion, low dose, long-term treatment of INS and GH to ageing rats improved the adrenergic, dopaminergic and serotonergic receptor subtypes activity and rejuvenation of brain function.
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Supported by grants from DST, DBT, ICMR, Govt. of India, and KSCSTE, Govt. of Kerala to Dr. C. S. Paulose. B. Savitha thanks Cochin University of Science and Technology for SRF.
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Paulose, C.S., Balakrishnan, S. Adrenergic, dopaminergic and serotonergic gene expression in low dose, long time insulin and somatotropin treatment to ageing rats: rejuvenation of brain function. Biogerontology 9, 429–439 (2008). https://doi.org/10.1007/s10522-008-9183-1
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DOI: https://doi.org/10.1007/s10522-008-9183-1