We studied immunolocalization of CD29, CD44, osteocalcin, and TGF-β1 in the bone tissue of the mandible of miniature pigs with extra-bone fixation of a free gingival graft. Three months after surgery, neoosteogenesis foci with high expression of the studied markers were found in the contact area of the free gingival graft with the alveolar bone. The markers were localized in the layer of external circumferential lamellae, on the surface of concentric lamellae of the growing osteons, and in the connective tissue of the Haversian canals. TGF-β1-immunopositive cells predominated in the connective tissue of the Haversian and Volkmann canals and in the adventitia and inner lining of the vascular wall. The established morphochemical patterns of osteogenous cells indicate significant reparative capabilities of a free gingival graft and allows considering it as an effective osteoinductive factor.
Similar content being viewed by others
References
Edranov SS, Kerzikov RA. Free gingival graft morphogenesis. Ross. Stomatol. Zh. 2017;21(2):111-116. Russian.
Kalinichenko SG, Matveeva NY, Kostiv RE, Puz’ AV. Role of Vascular Endothelial Growth Factor and Transforming Growth Factor-β2 in Rat Bone Tissue after Bone Fracture and Placement of Titanium Implants with Bioactive Bioresorbable Coatings. Bull. Exp. Biol. Med. 2017;162(5):671-675. doi: https://doi.org/10.1007/s10517-017-3684-3
Kostiv RE, Kalinichenko SG, Matveeva NYu. Trophic factors of bone growth, their morphogenetic characterization and clinical significance. Tikhookean. Med. Zh. 2017;(1):10-16. Russian.
Aydin S, Şahin F. Stem Cells Derived from Dental Tissues. Adv. Exp. Med. Biol. 2019;1144:123-132. doi: https://doi.org/10.1007/5584_2018_333
Cheon GB, Kang KL, Yoo MK, Yu JA, Lee DW. Alveolar Ridge Preservation Using Allografts and Dense Polytetrafluoroethylene Membranes With Open Membrane Technique in Unhealthy Extraction Socket. J. Oral. Implantol. 2017;43(4):267-273. doi: https://doi.org/10.1563/aaid-joi-D-17-00012
Franchi M, Orsini E, Trire A, Quaranta M, Martini D, Piccari GG, Ruggeri A, Ottani V. Osteogenesis and morphology of the peri-implant bone facing dental implants. ScientificWorld-Journal. 2004;4:1083-1095. doi: https://doi.org/10.1100/tsw.2004.211
Fournier BP, Ferre FC, Couty L, Lataillade JJ, Gourven M, Naveau A, Coulomb B, Lafont A, Gogly B. Multipotent progenitor cells in gingival connective tissue. Tissue Eng. Part A. 2010;16(9):2891-289. doi: https://doi.org/10.1089/ten.TEA.2009.0796
Grawish ME. Gingival-derived mesenchymal stem cells: An endless resource for regenerative dentistry. World J. Stem Cells. 2018;10(9):116-118. doi: https://doi.org/10.4252/wjsc.v10.i9.116
Hatzimanolakis P, Tsourounakis I, Kelekis-Cholakis A. Dental Implant Maintenance for the Oral Healthcare Team. Compend. Contin. Educ. Dent. 2019;40(7):424-429.
Iorio-Siciliano V, Blasi A, Sammartino G, Salvi GE, Sculean A. Soft tissue stability related to mucosal recession at dental implants: a systematic review. Quintessence Int. 2020;51(1):28-36. doi: https://doi.org/10.3290/j.qi.a43048
Kalinichenko SG, Matveeva NY, Kostiv RY, Edranov SS. The topography and proliferative activity of cells immunoreactive to various growth factors in rat femoral bone tissues after experimental fracture and implantation of titanium implants with bioactive biodegradable coatings. Biomed. Mater Eng. 2019;30(1):85-95. doi: https://doi.org/10.3233/BME-181035
Ladwein C, Schmelzeisen R, Nelson K, Fluegge TV, Fretwurst T. Is the presence of keratinized mucosa associated with periimplant tissue health? A clinical cross-sectional analysis. Int. J. Implant Dent. 2015;1(1):11. doi: https://doi.org/10.1186/s40729-015-0009-z
Monje A, Galindo-Moreno P, Tözüm TF, Suárez-López del Amo F, Wang HL. Into the Paradigm of Local Factors as Contributors for Peri-implant Disease: Short Communication. Int. J. Oral Maxillofac. Implants. 2016;31(2):288-292. doi: https://doi.org/10.11607/jomi.4265
Puisys A, Linkevicius T. The influence of mucosal tissue thickening on crestal bone stability around bone-level implants. A prospective controlled clinical trial. Clin. Oral Implants Res. 2015;26(2):123-129. doi: https://doi.org/10.1111/clr.12301
Sivaraj KK, Adams RH. Blood vessel formation and function in bone. Development. 2016;143(15):2706-2715. doi: https://doi.org/10.1242/dev.136861
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 3, pp. 391-396, March, 2021
Rights and permissions
About this article
Cite this article
Edranov, S.S., Matveeva, N.Y. & Kalinichenko, S.G. Osteogenic and Regenerative Potential of Free Gingival Graft. Bull Exp Biol Med 171, 404–408 (2021). https://doi.org/10.1007/s10517-021-05237-w
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05237-w