First to fourth-order branches of the uterine artery in sexually mature female Wistar rats were studied by biomicroscopy. After administration of a CO donor hemin (60 mM), the diameters of large uterine branches with a well-developed muscle layer markedly increased, while the increase in diameter of small vessels with one often interrupted layer of smooth muscle cells increased insignificantly. Zinc protoporphyrin IX (30 mM) in all cases blocked this effect. However, zinc protoporphyrin IX does not affect NO-mediated reaction of the branches of the uterine artery caused by administration of L-arginine (60 mM), and L-NAME did not significantly affect reactivity of uterine artery branches associated with the hemoxygenase—CO system. In contrast to NO, CO produced less potent and rapid, but more sustained effect. The target for the hemoxygenase—CO system is mainly arteries with developed muscular layer, while the target for the NO synthase—NO is small vessels where endothelium plays a Rdecisive role in the regulation of vasomotor reactions.
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Kotsyuba AE, Chertok VM, Chertok AG. Age-Specific Characteristics of CO-Mediated Reaction of the Pial Arteries of Various Diameters in Rats. Bull. Exp. Biol. Med. 2017;162(5):658-663. doi: https://doi.org/10.1007/s10517-017-3681-6
Khramova IA, Chertok VM, Kotsyuba AE, Chertok AG. Structural organization of the uterus circulatory system. Tikhookean. Med. Zh. 2018;(3):13-23. Russian.
Chertok VM, Kotsyuba AE. Immunolocation of Heme Oxygenases in the Walls of Cerebral Arteries of Various Diameters in Rats. Bull. Exp. Biol. Med. 2017;163(2):276-279. doi: https://doi.org/10.1007/s10517-017-3783-1
Chertok VM, Kotsyuba EP. Localization and Quantitative Assessment of Oxygen-Sensitive Hypoxia-Inducible Factor 1α in the Brain of the Mitten Crab Eriocheir Japonica in Normal Conditions and Acute Anoxia (an immunohistochemical study). Neurosc. Behav. Physiol. 2017;47(1):12-16.
Chertok VM, Nemkov YuK, Chertok AG. Intraorgan vasculature of the uterus. Vladivostok, 2018. Russian.
Andresen JJ, Shafi NI, Durante W, Bryan RM Jr. Effects of carbon monoxide and heme oxygenase inhibitors in cerebral vessels of rats and mice. Am. J. Physiol. Heart Circ. Physiol. 2006;291(1):H223-H230.
Fredenburgh LE, Merz AA, Cheng S. Haeme oxygenase signalling pathway: implications for cardiovascular disease. Eur. Heart J. 2015;36(24):1512-1518.
Gagov H, Kadinov B, Hristov K, Boev K, Itzev D, Bolton T, Duridanova D. Role of constitutively expressed heme oxygenase-2 in the regulation of guinea pig coronary artery tone. Pflugers Arch. 2003;446(4):412-421.
Leffler CW, Parfenova H, Jaggar JH. Carbon monoxide as an endogenous vascular modulator. Am. J. Physiol. Heart Circ. Physiol. 2011;301(1):H1-H11.
Levitt DG, Levitt MD. Carbon monoxide: a critical quantitative analysis and review of the extent and limitations of its second messenger function. Clin. Pharmacol. 2015;7:37-56.
Polizio AH, Santa-Cruz DM, Balestrasse KB, Gironacci MM, Bertera FM, Höcht C, Taira CA, Tomaro ML, Gorzalczany SB. Heme oxygenase-1 overexpression fails to attenuate hypertension when the nitric oxide synthase system is not fully operative. Pharmacology. 2011;87(5-6):341-349.
Tiwari S, Ndisang JF. Heme oxygenase system and hypertension: a comprehensive insight. Curr. Pharm. Des. 2014; 20(9):1354-1369.
Ushiyama M, Morita T, Katayama S. Carbon monoxide regulates blood pressure cooperatively with nitric oxide in hypertensive rats. Heart Vessels. 2002;16(5):189-195.
Wesseling S, Fledderus JO, Verhaar MC, Joles JA. Beneficial effects of diminished production of hydrogen sulfide or carbon monoxide on hypertension and renal injury induced by NO withdrawal. Br. J. Pharmacol. 2015;172(6):1607-1619.
Wu ML, Ho YC, Lin CY, Yet SF. Heme oxygenase-1 in inflammation and cardiovascular disease. Am. J. Cardiovasc. Dis. 2011;1(2):150-158.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 6, pp. 767-771, June, 2019
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Kotsyuba, A.E., Chertok, V.M. & Khramova, I.A. Effect of the Hemoxygenase—Carbon Monoxide (HO—CO) System on the Reactivity of Uterine Artery Branches in Rats. Bull Exp Biol Med 167, 805–808 (2019). https://doi.org/10.1007/s10517-019-04627-5
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DOI: https://doi.org/10.1007/s10517-019-04627-5