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Non-Invasive Evaluation of Extracellular Matrix Formation in the Intestinal Epithelium

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Differentiation of colorectal cancer Caco-2 cells was assessed using Affymetrix Human Gene 1.0 ST arrays and by the main electrical parameters measured by bioimpedance spectroscopy. Transepithelial electrical resistance (TEER) was maximum on day 7, then decreased by day 11, and remained stable. The baseline resistance was maximum on day 4, minimum on day 7, but then gradually increased over 2 weeks, which can be explained by the formation of the basement membrane components or the apical mucous layer. Caco-2 cells express components of laminin-111 and laminin-511. A synchronous increase in the expression of mucin 3 mRNA (MUC3A/MUC3B) and mucin 17 mRNA (MUC17) and reduced expression of miR-21 and miR-622 microRNA genes were observed. Possible use of the described approach for studying the formation of extracellular matrix is discussed.

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Authors and Affiliations

  1. BioClinicum Research Center, Moscow, Russia

    S. V. Nikulin, E. N. Knyazev, T. N. Gerasimenko, S. A. Shilin, I. N. Gazizov & G. S. Zakharova

  2. Moscow Institute of Physics and Technology (MIPT), Moscow, Russia

    S. V. Nikulin

  3. National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, Russia

    A. A. Poloznikov & M. Yu. Shkurnikov

Authors
  1. S. V. Nikulin
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  2. E. N. Knyazev
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  3. T. N. Gerasimenko
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  4. S. A. Shilin
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  5. I. N. Gazizov
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  6. G. S. Zakharova
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  7. A. A. Poloznikov
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  8. M. Yu. Shkurnikov
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Correspondence to S. V. Nikulin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 7, pp. 41-45, July, 2018

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Nikulin, S.V., Knyazev, E.N., Gerasimenko, T.N. et al. Non-Invasive Evaluation of Extracellular Matrix Formation in the Intestinal Epithelium. Bull Exp Biol Med 166, 35–38 (2018). https://doi.org/10.1007/s10517-018-4283-7

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  • DOI: https://doi.org/10.1007/s10517-018-4283-7

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