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Enzyme Changes in the Offspring of Female Rats due to Long-Term Administration of Cyclic AMP and Insulin before Pregnancy

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effects of insulin and cAMP on the offspring of female rats after daily treatment with these substances over 4 weeks. In adult offspring from cAMP-treated females, activities of pyruvate kinase and glucose-6-phosphate dehydrogenase decreased in the liver and brain and activities of NADP-dependent malate dehydrogenase and 6-phosphogluconate dehydrogenase decreased in the liver. In the offspring of insulin-treated females, we observed only activation of glucose-6-phosphate dehydrogenase and malate dehydrogenase in the liver and only in females. Enzyme activity probably correlates with their content, as no changes in their kinetic properties were observed under these conditions. Long-term hormone treatment before pregnancy can affect the expression of genes for some enzymes in the offspring due to transmission of epigenetic signals by the ovum. However, further studies are required to confirm this mechanism.

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References

  1. Anway MD, Leathers C, Skinner MK. Endocrine disruptor vinclozolin induced epigenetic transgenerational adult-onset disease. Endocrinology. 2006; 147(12):5515-5523.

    Article  CAS  PubMed  Google Scholar 

  2. Berg JM, Tymoczko JL, Stryer L. Biochemistry. Stanford, 2012.

  3. Bernstein BE, Meissner A, Lander ES. The mammalian epigenome. Cell. 2007;128(4):669-6681.

    Article  CAS  PubMed  Google Scholar 

  4. Bisswanger H. Practical Enzymology. Weinheim, 2002.

  5. Czyzewska U, Tylicki A, Siemieniuk M, Strumilo S. Changes of activity and kinetics of certain liver and heart enzymesof hypothyroid and T(3)-treated rats. J. Physiol. Biochem. 2012;68(3):345-351.

    Article  CAS  PubMed  Google Scholar 

  6. Filkowski JN, Ilnytskyy Y, Tamminga J, Koturbash I, Golubov A, Bagnyukova T, Pogribny IP, Kovalchuk O. Hypomethylation and genome instability in the germline of exposed parents and their progeny is associated with altered miRNA expression. Carcinogenesis. 2010; 31(6):1110-1115.

    Article  CAS  PubMed  Google Scholar 

  7. Jablonka E, Raz G. Transgenerational epigenetic inheritance: prevalence, mechanisms, and implications for the study of heredity and evolution. Q Rev. Biol. 2009; 84(2):131-176.

    Article  PubMed  Google Scholar 

  8. Kaati G, Bygren LO, Pembrey M, Sjöström M. Transgenerational response to nutrition, early life circumstances and longevity. Eur. J. Hum. Genet. 2007; 15(7):784-790.

    Article  CAS  PubMed  Google Scholar 

  9. Morgan HD, Santos F, Green K, Dean W, Reik W. Epigenetic reprogramming in mammals. Hum. Mol. Genet. 2005; 14(Spec No 1):R47-R58.

  10. Ng SF, Lin RC, Laybutt DR, Barres R, Owens JA, Morris MJ. Chronic high-fat diet in fathers programs b-cell dysfunction in female rat offspring. Nature. 2010; 467:963-966.

    Article  CAS  PubMed  Google Scholar 

  11. Pavlova MB, Savenko YN, Dyuzhikova NA, Shiryaeva NV, Vaido AI. Effect of chronic emotional and pain stress on histone H3 phosphorylation in the hippocampus of rat strains with different excitability of the nervous system. Bull. Exp. Biol. Med. 2012; 153(3):357-360.

    Article  CAS  PubMed  Google Scholar 

  12. Skinner MK, Manikkam M, Guerrero-Bosagna C. Epigenetic transgenerational actions of environmental factors in disease etiology. Trends Endocrinol. Metab. 2010; 21(4):214-222.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Smirnikhina SA, Voronina ES, Lavrov AV, Bochkov NP. Dioxidine-induced changes in genome-wide DNA methylation in a culture of peripheral blood lymphocytes. Bull. Exp. Biol. Med. 2013; 155(2):224-227.

    Article  CAS  PubMed  Google Scholar 

  14. Soubry A, Hoyo C, Jirtle RL, Murphy SK. A paternal environmental legacy: evidence for epigenetic inheritance through the male germ line. Bioessays. 2014; 36(4):359-371.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Tylicki A, Kawalko A, Sokolska J, Strumilo S. Effect of anabolic steroid nandrolone decanoate on the properties of certain enzymes in the heart, liver, and muscle of rats, and their effect on rats’ cardiac electrophysiology. Horm. Metab. Res. 2007; 39(4):268-272.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Biology, University of Białystok, Białystok, Poland

    S. A. Strumilo, U. Czyzewska, M. Siemieniuk, J. Strumilo & A. Tylicki

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  1. S. A. Strumilo
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  2. U. Czyzewska
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  3. M. Siemieniuk
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  4. J. Strumilo
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  5. A. Tylicki
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Correspondence to S. A. Strumilo.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 3, pp. 352-354, March, 2016

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Strumilo, S.A., Czyzewska, U., Siemieniuk, M. et al. Enzyme Changes in the Offspring of Female Rats due to Long-Term Administration of Cyclic AMP and Insulin before Pregnancy. Bull Exp Biol Med 161, 374–376 (2016). https://doi.org/10.1007/s10517-016-3417-z

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  • DOI: https://doi.org/10.1007/s10517-016-3417-z

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