We analyzed the dynamics of neuropathic pain development and changes in catalase and superoxide dismutase (SOD) activities in the brain, liver, and skeletal muscles of male Wistar rats with 1-month streptozotocin-induced diabetes mellitus. A decrease in mechanical nociceptive threshold was revealed that progressed during the disease progress. Insulin treatment restored nociceptive threshold in diabetic animals to the control values. Catalase activity in the liver and skeletal muscles of diabetic rats increased by 1.5 and 2 times, respectively, in comparison with the control, while insulin treatment reduced enzyme activity to the control level. In the brain, catalase activity was reduced by 1.5 times and insulin therapy did affect this parameter. SOD activity in the studied tissues remained unchanged during diabetes and was not affected by insulin therapy. A strong negative correlation between nociceptive threshold in rats and catalase activity in their liver and skeletal muscles was found.
Similar content being viewed by others
References
H. Aebi, Catalase in vitro. Methods Enzymol., 105, 121-126 (1984).
N. Altan, S. Yiğit, E. Elmali, E. Malhatun, S. Rota, and N. Kiliç, Effect of the sulfonylurea glyburide on superoxide dismutase in streptozotocin-induced diabetic rat muscle. Gen. Pharmacol., 28, No. 5, 795-796 (1997).
T. Anwer, M. Sharma, K. K. Pillai, S. E. Haque, M. M. Alam, and M. S. Zaman, Protective effect of bezafibrate on streptozotocin-induced oxidative stress and toxicity in rats. Toxicology., 229, Nos. 1-2, 165-172 (2007).
B. C. Callaghan, H. T. Cheng, C. L. Stables, A. L. Smith, and E. L. Feldman, Diabetic neuropathy: clinical manifestations and current treatments. Lancet Neurol., 11, No. 6, 521-534 (2012).
L. B. Ceretta, G. Z. Réus, H. M. Abelaira, K. F. Ribeiro, G. Zappellini, F. F. Felisbino, A. V. Steckert, F. Dal-Pizzol, and J. Quevedo, Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan-induced diabetic rats. Exp. Diabetes Res., 2012, doi: 10.1155/2012/302682 (2012).
E. Feldman, Oxidative stress and diabetic neuropathy: a new understanding of an old problem. J. Clin. Invest., 111, No. 4, 431-433 (2003).
D. Fuchs, F. Birklein, P. W. Reeh, and S. K. Sauer, Sensitized peripheral nociception in experimental diabetes of the rat. Pain, 151, No. 2, 496-505 (2010).
R. E. Heikkila and F. Cabbat, A sensitive assay for superoxide dismutase based on the autoxidation of 6-hydroxydopamine. Anal. Biochem., 75, No. 2, 356-362 (1976).
R. Kakkar, J. Kalra, S. V. Mantha, and K. Prasad, Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol. Cell. Biochem., 151, No. 2, 113-119 (1995).
K. Kaul, J.M. Tarr, S. I. Ahmad, E. M. Kohner, and R. Chibber, Introduction to diabetes mellitus. Adv. Exp. Med. Biol., 771, 1-11 (2012).
O. Kurt, T. Y. Ozden, N. Ozsoy, S. Tunali, A. Can, N. Akev, and R. Yanardag, Influence of vanadium supplementation on oxidative stress factors in the muscle of STZ-diabetic rats. Biometals, 24, No. 5, 943-949 (2011).
L. Pari and M. Latha, Protective role of Scoparia dulcis plant extract on brain antioxidant status and lipid peroxidation in STZ diabetic male Wistar rats. BMC Complement. Altern. Med., 4, 16 (2004).
M. Ramanathan, A. K. Jaiswal, and S. K. Bhattacharya, Superoxide dismutase, catalase and glutathione peroxidase activities in the brain of streptozotocin induced diabetic rats. Indian J. Exp. Biol., 37, No. 2, 182-183 (1999).
D. Romanovsky, S. L. Hastings, J. R. Stimers, and M. Dobretsov, Relevance of hyperglycemia to early mechanical hyperalgesia in streptozotocin-induced diabetes. J. Peripher. Nerv. Syst., 9, No. 2, 62-69 (2004).
R. A. Sanders, F. M. Rauscher, and J. B. Watkins 3rd, Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. J. Biochem. Mol. Toxicol., 15, No. 3, 143-149 (2001).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 1, pp. 18-22, January, 2015
Rights and permissions
About this article
Cite this article
Shipilov, V.N., Chistyakova, O.V. & Trost, A.M. Relationships between Mechanical Nociceptive Threshold and Activity of Antioxidant Enzymes in Male Rats with Experimental Type I Diabetes Mellitus. Bull Exp Biol Med 161, 11–14 (2016). https://doi.org/10.1007/s10517-016-3333-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3333-2