Skip to main content
SpringerLink
Log in

Lipid peroxidation and activity of antioxidant enzymes in diabetic rats

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

We hypothesized that oxygen free radicals (OFRs) may be involved in pathogenesis of diabetic complications. We therefore investigated the levels of lipid peroxidation by measuring thiobarbituric acid reactive substances (TBARS) and activity of antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] in tissues and blood of streptozotocin (STZ)-induced diabetic rats. The animals were divided into two groups: control and diabetic. After 10 weeks (wks) of diabetes the animals were sacrificed and liver, heart, pancreas, kidney and blood were collected for measurement of various biochemical parameters. Diabetes was associated with a significant increase in TBARS in pancreas, heart and blood. The activity of CAT increased in liver, heart and blood but decreased in kidney. GSH-Px activity increased in pancreas and kidney while SOD activity increased in liver, heart and pancreas. Our findings suggest that oxidative stress occurs in diabetic state and that oxidative damage to tissues may be a contributory factor in complications associated with diabetes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Oberley LW: Free radicals and diabetes. Free Rad Biol Med 5: 113–124, 1988

    Google Scholar 

  2. Baynes JW: Perspectives in diabetes, Role of oxidative stress in development of complications in Diabetes. Diabetes 40: 405–412, 1991

    Google Scholar 

  3. Asplund K, Grankvist K, Marklund S, Taljedal IB: Partial protection against streptozotocin induced hyperglycemia by superoxide dismutase linked to polyethylene glycol. Acta Endocrinol 107: 390–394, 1984

    Google Scholar 

  4. Asayama K, English D, Slonim AE, Buer IM: Chemiluminescence as an index of drug induced free radical production in pancreatic islets. Diabetes 33: 160–163, 1984

    Google Scholar 

  5. Hunt JV, Smith CCT, Wolff SP: Autoxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose. Diabetes 39: 1420–1424, 1990

    Google Scholar 

  6. Wolff SP, Dean RT: Glucose autoxidation and protein modification the potential role of autoxidative glycosylation in diabetes. Biochem J 245: 243–250, 1987

    Google Scholar 

  7. Halliwell B, Gutteridge JMC: Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet 1: 1396–1397, 1984

    Google Scholar 

  8. Meerson FZ, Kagan VE, Kozlov YP, Belkina LM, Arkhipenko YV: The role of lipid peroxidation in pathogenesis of ischemic damage and antioxidant protection of the heart. Basic Res Cardiol 77: 465–468, 1982

    Google Scholar 

  9. Salin ML, McCord JM: Superoxide dismutases in polymorphonuclear leukocytes. J Clin Invest 54: 1005–1009, 1974

    Google Scholar 

  10. Freeman BA, Crapo JD: Biology of disease: Free radicals and tissue injury. Lab Invest 47: 412–426, 1982

    Google Scholar 

  11. McCord JM: Oxygen derived free radicals in post-ischemic tissue injury. N Eng J Med 312: 159–163, 1985

    Google Scholar 

  12. Mantha SV, Prasad M, Kalra J, Prasad K: Antioxidant enzymes in hypercholesterolemia and effects of Vitamin E in rabbits. Atherosclerosis 101: 135–144, 1993

    Google Scholar 

  13. Dodson PM: Dyslipidemia and diabetes: an introduction. In: P.M. Dodson and A.H. Barnett (eds.). Lipids, diabetes and vascular disease, Science press Limited, London, 1992, pp. 1–15

    Google Scholar 

  14. Morel DW, Hessler JR, Chisolm GM: Low density lipoprotein cytotoxicity induced by free radical peroxidation of lipid. Lipid Res 24: 1070–1076, 1983

    Google Scholar 

  15. Matkovics B, Varga SI, Szabo L, Witas H: The effect of diabetes on the activities of the peroxide metabolizing enzymes. Horm Metab Res 14: 77–79, 1983

    Google Scholar 

  16. Like AA, Rossini A: Streptozotocin-induced pancreatic insulitis: new model of diabetes mellitus. Science 193: 415–417, 1976

    Google Scholar 

  17. Prasad K, Lee P, Mantha SV, Kalra J, Prasad M, Gupta JB: Detection of ischemia-reperfusion cardiac injury by cardiac muscle chemiluminescence. Mol Cell Biochem 115: 49–58, 1992

    Google Scholar 

  18. Kadish AH, Little RL, Sternberg JC: A new and rapid method for the determination of glucose by measurement of rate of oxygen consumption. Clin Chem 14: 116–131, 1968

    Google Scholar 

  19. Yagi K: Assay for blood plasma or serum. In: L. Packer (ed.). Methods in Enzymology. Academic press, New York, Vol. 105, 1984, pp. 328–331

    Google Scholar 

  20. Aebi H: Catalase. In: Methods of enzymatic analysis. Bergmeyer HV (Ed.), Chemie, Weinheim, F.R.G., pp. 673–684, 1974

    Google Scholar 

  21. Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158–169, 1967

    Google Scholar 

  22. Lawrence RA, Burk RF: Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Commun 71: 952–958, 1976

    Google Scholar 

  23. Sun Y, Peterson TE, McCormick ML, Oberley LW, Osborne JW: Improved superoxide dismutase assay for clinical use. Clin Chem 35: 1265–1266, 1989

    Google Scholar 

  24. Gornall AG, Bardwill CJ, David MM: Determination of serum proteins by means of biuret reaction. J Biol Chem 177: 751–766, 1949

    Google Scholar 

  25. Eilers RJ: Notification of final adoption of an international method and standard solution for haemoglobinometry. Specifications for preparation of standard solution. Am J Clin Pathol 47: 212–214, 1967

    Google Scholar 

  26. Malaisse WJ: Alloxan toxicity to the pancreatic B-cell. Biochem Pharmacol 31: 3527–3534, 1982

    Google Scholar 

  27. Sato Y, Hotta N, Sakamoto N et al.: Lipid peroxide level in plasma of diabetic patients. Biochem Med 25: 373–378, 1981

    Google Scholar 

  28. Takasu N, Komiya I, Asasa T, Nagasawa Y, Yamada T: Streptozotocin and Alloxan induced H2O2 generation and DNA fragmentation in pancreatic islets. Diabetes 40: 1141–1145, 1991

    Google Scholar 

  29. Tjalve H, Wilander E, Johansson E: Distribution of labelled streptozotocin in mice: uptake and retention in pancreatic islets. J Endocrinol 69: 455–456, 1976

    Google Scholar 

  30. Wohaeib SA, Godin DV: Starvation related alterations in free radical defense mechanisms in rats. Diabetes 36: 169–173, 1987

    Google Scholar 

  31. Horie S, Ishii H, Suga T: Changes in peroxisomal fatty acid oxidation in diabetic rat liver. J Biochem 90: 1691–1696, 1981

    Google Scholar 

  32. Aust SD, Morehouse LA, Thomas CE: Role of metals in oxygen radical reaction. J Free Rad Biol Med 1: 3–25, 1985

    Google Scholar 

  33. Halliwell JMC, Gutteridge JMC: Role of free radicals and catalytic metal ions in human disease: An overview. In: L. Packer and A.N. Glazer (eds). Methods in Enzymolgy, Academic press Inc., San Diego, 1990, vol. 186, pp 1–25

    Google Scholar 

  34. Srivastava P, Saxena AK, Kale RK, Baquer NZ: Insulin like effects of lithium and vanadate on the altered antioxidant status of diabetic rats. Res Commun Chem Pathol Pharmacol 80: 283–293, 1993

    Google Scholar 

  35. Kono Y, Fridovich I: Superoxide radicals inhibit catalase. J Biol Chem 257: 5751–5754, 1982

    Google Scholar 

  36. De duve C, Baudhhuin P: Peroxisomes (Microbodies and related particles). Physiol Rev 46: 323–341, 1966

    Google Scholar 

  37. Doroshow JH, Lockes GY, Myers GE: Enzymatic defences of the mouse heart against reactive oxygen metabolites. J Clin Invest 65: 128–135, 1980

    Google Scholar 

  38. Frank L, Messaro D: Oxygen toxicity. Am J Med 69: 117–126, 1980

    Google Scholar 

  39. Oei HH, Stroo WE, Burton KP, Schaffer SW: A possible role of xanthine oxidase in producing oxidative stress in heart of chronically ethanol treated rats. Res Commun Chem Pathol Pharmacol 38: 453, 1982

    Google Scholar 

  40. Shull S, Heintz NH, Periasamy M, Manohar M, Janssen YM, Marsh JP, Mossman BT: Differential regulation of antioxidant enzymes in response to oxidants. J Biol Chem 266: 24398–24403, 1991

    Google Scholar 

  41. Bray RC, Cockle SA, Martin-Fielder E, Roberts PB, Rotillo G, Calabrase L: Reduction and inactivation of superoxide dismutase by hydrogen peroxide. Biochem J 139: 43–48, 1974

    Google Scholar 

  42. Kono Y, Fridovich I: Inhibition and reactivation of Mn-catalase. J Biol Chem 258: 13646–13648, 1983

    Google Scholar 

  43. Blum J, Fridovich I: Inactivation of glutathione peroxidase by superoxide radicals. Arch Biochem Biophys 240: 500–508, 1985

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, College of Medicine, University of Saskatchewan and Royal University Hospital, S7N OW8, Saskatoon, Saskatchewan, Canada

    Rakesh Kakkar, Jawahar Kalra, Subrahmanyam V. Mantha & Kailash Prasad

  2. Department of Physiology, College of Medicine, University of Saskatchewan and Royal University Hospital, S7N OW8, Saskatoon, Saskatchewan, Canada

    Rakesh Kakkar, Jawahar Kalra, Subrahmanyam V. Mantha & Kailash Prasad

Authors
  1. Rakesh Kakkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jawahar Kalra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Subrahmanyam V. Mantha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kailash Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kakkar, R., Kalra, J., Mantha, S.V. et al. Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol Cell Biochem 151, 113–119 (1995). https://doi.org/10.1007/BF01322333

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01322333

Key Words

Search

Navigation