Parameters of respiration, transmembrane potential, and Ca2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 156, No. 11, pp. 589-592, November, 2013
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Lishmanov, Y.B., Naryzhnaya, N.V., Maslov, L.N. et al. Functional State of Myocardial Mitochondria in Ischemia Reperfusion of the Heart in Rats Adapted to Hypoxia. Bull Exp Biol Med 156, 645–648 (2014). https://doi.org/10.1007/s10517-014-2416-1
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DOI: https://doi.org/10.1007/s10517-014-2416-1