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Profiling drug-induced cell death pathways in the zebrafish lateral line

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Abstract

Programmed cell death (PCD) is an important process in development and disease, as it allows the body to rid itself of unwanted or damaged cells. However, PCD pathways can also be activated in otherwise healthy cells. One such case occurs in sensory hair cells of the inner ear following exposure to ototoxic drugs, resulting in hearing loss and/or balance disorders. The intracellular pathways that determine if hair cells die or survive following this or other ototoxic challenges are incompletely understood. We use the larval zebrafish lateral line, an external hair cell-bearing sensory system, as a platform for profiling cell death pathways activated in response to ototoxic stimuli. In this report the importance of each pathway was assessed by screening a custom cell death inhibitor library for instances when pathway inhibition protected hair cells from the aminoglycosides neomycin or gentamicin, or the chemotherapy agent cisplatin. This screen revealed that each ototoxin likely activated a distinct subset of possible cell death pathways. For example, the proteasome inhibitor Z-LLF-CHO protected hair cells from either aminoglycoside or from cisplatin, while d-methionine, an antioxidant, protected hair cells from gentamicin or cisplatin but not from neomycin toxicity. The calpain inhibitor leupeptin primarily protected hair cells from neomycin, as did a Bax channel blocker. Neither caspase inhibition nor protein synthesis inhibition altered the progression of hair cell death. Taken together, these results suggest that ototoxin-treated hair cells die via multiple processes that form an interactive network of cell death signaling cascades.

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Acknowledgments

This research was funded by the National Institute on Deafness and Other Communication Disorders (NIDCD) Grants DC004661, DC005987 (D.W.R. and E.W R.), DC009931 (A.B.C.), and DC011344 (A.B.C.). Support was provided by the Virginia Merrill Bloedel Hearing Research Center. We thank several anonymous reviewers for their comments that helped strengthen this manuscript.

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Authors and Affiliations

  1. Virginia Merrill Bloedel Hearing Research Center, University of Washington, Box 357923, Seattle, WA, 98195, USA

    Allison B. Coffin, Kay L. Williamson, David W. Raible & Edwin W. Rubel

  2. Department of Otolaryngology–Head and Neck Surgery, University of Washington, Box 356515, Seattle, WA, 98195, USA

    Allison B. Coffin, Kay L. Williamson & Edwin W. Rubel

  3. Department of Speech and Hearing Science, University of Washington, Box 354875, Seattle, WA, 98105, USA

    Anna Mamiya

  4. Department of Biological Structure, University of Washington, Box 357420, Seattle, WA, 98195, USA

    David W. Raible

  5. Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Arts and Sciences, Washington State University, Vancouver, WA, 98686, USA

    Allison B. Coffin & Kay L. Williamson

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  1. Allison B. Coffin
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  2. Kay L. Williamson
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  3. Anna Mamiya
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  4. David W. Raible
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  5. Edwin W. Rubel
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Correspondence to Allison B. Coffin.

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Coffin, A.B., Williamson, K.L., Mamiya, A. et al. Profiling drug-induced cell death pathways in the zebrafish lateral line. Apoptosis 18, 393–408 (2013). https://doi.org/10.1007/s10495-013-0816-8

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  • DOI: https://doi.org/10.1007/s10495-013-0816-8

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