Abstract
The family Tetranychidae includes many agriculturally important species known as spider mites. Their morphological identification is quite difficult due to the tiny size of their taxonomic characters and the requirement for high-level expertise. This may lead to pest misidentification and thus failure in pest management. DNA-based species identification seems to offer an alternative solution to overcome these issues. In the present study, two common molecular markers—Cytochrome oxidase subunit I (COI) and Internal transcribed spacer 2 (ITS2)—were used to identify 10 spider mite species from Turkey. Furthermore, genetic distances for several of them were assessed. Panonychus ulmi and Bryobia kissophila had the lowest (1.1%) and highest (4.5%) intra-specific genetic distances, respectively. In addition, integrative taxonomy allowed to identify Eotetranychus quercicola in Turkey as a new record. The sequences herein obtained will allow rapid species identification using molecular techniques and will contribute to resolve the phylogenetic history of spider mites.
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Acknowledgements
The authors would like to thank to everyone who provides plant samples. This study is a part of the PhD thesis of first author.
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This research was supported by Ankara University Scientific Research Projects Coordination Unit. Project Number 19L0447009, 2021.
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Eİ and SÇ: conceived the ideas. Eİ: performed the experiments. All the authors analysed the data. Eİ, PA and AM: wrote the main manuscript text. All authors read and approved the final manuscript.
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İnak, E., Çobanoğlu, S., Auger, P. et al. Molecular identification and phylogenetic analysis of spider mites (Prostigmata: Tetranychidae) of Turkey. Exp Appl Acarol 87, 195–205 (2022). https://doi.org/10.1007/s10493-022-00728-5
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DOI: https://doi.org/10.1007/s10493-022-00728-5