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Terrihabitans rhizophilus sp. nov., isolated from the rhizosphere soil of plant in temperate semi-arid steppe

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Abstract

A bacterial strain PJ23T was isolated from the rhizosphere soil of Elymus dahuricus Turcz. sampled from a temperate semi-arid steppe in the northern of Inner Mongolia Autonomous Region, China. The strain is Gram-stain-negative, aerobic, light-pink, short rod-shaped, and non-spore-forming. Cell growth could be observed at 4–29℃ (optimal at 24℃), pH 6.0–8.6 (optimal at 8.0) and in the presence of 0–5.0% (w/v) NaCl (optimal at 2.5%). The major cellular fatty acids of strain PJ23T were Summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) (39.42%) and C16:0 (9.60%). The polar lipids were phosphatidylcholine, two unidentified glycolipids, one unidentified aminophospholipid, and two other unidentified polar lipids. The major respiratory quinone was ubiquinone-10. Phylogeny analysis based on 16S rRNA gene sequences retrieved from the genomes showed that, the strain was closely related to the species Terrihabitans soli IZ6T and Flaviflagellibacter deserti SYSU D60017T, with the sequence similarities of 96.79% and 96.15%, respectively. The G + C content was 65.23 mol% calculated on draft genome sequencing. Between the strains PJ23T and Terrihabitans soli IZ6T, the average nucleotide identity (ANI), amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) was 73.39%,71.12% and 15.7%, these values were lower than the proposed and generally accepted species boundaries of ANI, AAI and dDDH, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic characteristics, strain PJ23T represents a novel species of Terrihabitans, for which the name Terrihabitans rhizophilus sp. nov. is proposed. The type strain is PJ23T (= KCTC 92977 T = CGMCC 1.61577 T).

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Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

R2A:

Reasoner’s 2A

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Funding

This work was mainly supported by the Applied Technology Research and Development Fund of Inner Mongolia (2021GG0360), Natural Science Foundation of Inner Mongolia Autonomous Region (2023LHMS03030 and 2023MS03016) and Science and Technology Program of Inner Mongolia Autonomous Region (2023YFHH0028).

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Author notes

  1. Runze Bao and Huiling Guo contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Environmental Microbiology and Biotechnology in Arid and Cold Regions, College of Life Science, Inner Mongolia Agricultural University, Huhhot, 010018, PR, China

    Runze Bao, Huiling Guo, Yungang Liang, Kai Tang, Fuying Feng & Jianyu Meng

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  1. Runze Bao
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  2. Huiling Guo
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  3. Yungang Liang
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  4. Kai Tang
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Contributions

Runze Bao, Kai Tang and Fuying Feng wrote the main manuscript text, Huiling Guo organized the data, while Yungang Liang prepared Figures 1 and S1-S4. All authors reviewed the manuscript.

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Correspondence to Jianyu Meng.

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The GenBank/ENA/DDBJ accession numbers for 16S rRNA gene sequence and the draft genome of Terrihabitans rhizophilus strain PJ23T are OR801651 and JAXAFJ000000000, respectively.

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Bao, R., Guo, H., Liang, Y. et al. Terrihabitans rhizophilus sp. nov., isolated from the rhizosphere soil of plant in temperate semi-arid steppe. Antonie van Leeuwenhoek 117, 67 (2024). https://doi.org/10.1007/s10482-024-01966-w

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