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Abundant and diverse endophytic bacteria associated with medicinal plant Arctium lappa L. and their potential for host plant growth promoting

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Abstract

Arctium lappa L. is one of the medicinal and food homologous plants in China, which is rich in nutrients and medicinal ingredients. The use of plant growth-promoting (PGP) endophytic bacteria is an alternative to reducing chemical fertilizers in agricultural production. The aim of this study was to analyze the diversity of endophytic bacteria in different cultivars of A. lappa L. collected from two geographical locations in China and evaluate PGP traits of the isolates and their potential PGP ability in greenhouse condition. Endophytic bacterial community was investigated by culture-dependent and culture-independent methods. Isolates were screened and investigated for multiple PGP traits, and representative strains were inoculated host seedlings to evaluate the growth promoting effect. A total of 348 endophytic bacteria were obtained and they were distributed into 4 phyla and 30 genera. In addition, high throughput sequencing revealed more abundant bacterial community, including 17 bacterial phyla and 207 genera. A high proportion of PGP traits were detected, including production of indole acetic acid, siderophore, ammonia and phosphate solubilization. Four representative strains with multiple PGP traits of the most dominant genera (Bacillus, Pantoea, Microbacterium and Pseudomonas) were further selected for host inoculation and growth promoting evaluation, and they significantly increase seedlings length, root length and fresh weight. This study demonstrated that A. lappa L. harbors abundant endophytic bacteria, and some endophytic bacteria showed good potential for the development of microbial fertilizer in the future.

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Acknowledgements

This work was supported by the Promoting Science and Technology Innovation Project of Xuzhou City (KC21130), North Jiangsu Science and Technology Special Project of Jiangsu Province (XZ-SZ202146), Natural Science Foundation of the Jiangsu Higher Education Institutions of China (17KJA180004).

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  1. Jia-Qi Liu and Shu-Mei Chen contributed equally to this work.

Authors and Affiliations

  1. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, People’s Republic of China

    Jia-Qi Liu, Shu-Mei Chen, Chun-Mei Zhang, Ming-Jie Xu, Ke Xing & Sheng Qin

  2. Xuzhou Kuaibang Biotechnology Development Co., Ltd, Xuzhou, Jiangsu, People’s Republic of China

    Cheng-Guo Li

  3. Jiangsu Runzhong Agricultural Technology Co., Ltd, Xinyi, Jiangsu, People’s Republic of China

    Kui Li

  4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China

    Yu-Qin Zhang

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Contributions

J-QL: Investigation, Methodology, Data curation, Formal analysis. S-MC: Methodology, Data curation, Formal analysis. C-MZ: Writing—review & editing. M-JX: Data curation, Formal analysis. KX: Writing—review & editing. Cheng-Guo Li: Investigation. KL: Sampling. Y-QZ: Methodology, Formal analysis. SQ: Conceptualization, Project administration, Supervision, Funding acquisition, Writing—review & editing.

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Liu, JQ., Chen, SM., Zhang, CM. et al. Abundant and diverse endophytic bacteria associated with medicinal plant Arctium lappa L. and their potential for host plant growth promoting. Antonie van Leeuwenhoek 115, 1405–1420 (2022). https://doi.org/10.1007/s10482-022-01785-x

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