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Description of Lujinxingia vulgaris sp. nov., isolated from coastal sediment via prey-traps

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Abstract

Two Gram-stain negative, facultative anaerobic, oxidase-negative, catalase-positive bacilli, designated as strains TMQ4T and TMQ2, were isolated from Xiaoshi Island, China, using prey-traps. Growth was observed within the ranges 25–45 °C (optimally at 37 °C), pH 6.5–9.0 (optimally at pH 7.5–8.0) and 1–8% NaCl (optimally at 3–4%, w/v). The draft genome sequences of strains TMQ4T and TMQ2 contained 184 contigs of 5,609,735 bp with a G+C content of 64.4% and 148 contigs of 5,589,985 bp with a G+C content of 65.0%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains belonged to the genus Lujinxingia with the similarity of 98.9%. The phylogenetic and phylogenomic topologies and analyses demonstrated that both strains clustered together and differentiated from the closest neighbour, Lujinxingia sediminis SEH01T. Genomic analyses showed that two strains lost the biosynthesis pathway of several chemical compounds. Iso-C15:0 was contained in the predominant cellular fatty acids in both strains. The major polar lipids of both strains consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified lipids; and the respiratory quinone was menaquinone MK-7 for both strains. Both strains predated other bacteria, including Owenweeksia hongkongensis JCM 12287T and Paraliobacillus ryukyuensis DSM 15140T, and were lured with one prey Acinetobacter baumannii ATCC 19606T in prey-trap. Combining genomic analyses, two strains had the predatory indices of 2, similar to representative typical bacterial predators. The physiological, biochemical, and phylogenetic properties suggest that the two strains represent a novel species within the genus Lujinxingia. The name Lujinxingia vulgaris sp. nov. is proposed, with strain TMQ4T (= KCTC 62851T = MCCC 1H00392T) as type strain and strain TMQ2 (= KCTC 72,079 = MCCC 1H00381) as reference strain.

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Abbreviations

GTDB:

Genome Taxonomy Database

HPLC:

High Performance Liquid Chromatography

MA:

Marine agar 2216

MB:

Marine broth 2216

MCCC:

Marine Culture Collection of China

MEGA:

Molecular Evolutionary Genetics Analysis

MIDI:

Microbial Identification System

NCBI:

National Centre of Biotechnology Information

RAST:

Rapid Annotation using Subsystem Technology

TLC:

Thin layer chromatography

UBCG:

Up-to-date Bacterial Core Gene Set

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Acknowledgements

This work of scanning electron microscope was supported by the Physical-Chemical Materials Analytical & Testing Centre of Shandong University at Weihai. We appreciate Prof. Aharon Oren for his generous help in Latin Nomenclature.

Funding

This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).

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Authors and Affiliations

  1. Marine College, Shandong University, Weihai, 264209, Shandong, China

    Shuo Wang, Da-Shuai Mu & Zong-Jun Du

  2. Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, Fujian, China

    Guang-Yu Li

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  1. Shuo Wang
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  2. Da-Shuai Mu
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  3. Guang-Yu Li
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  4. Zong-Jun Du
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Correspondence to Zong-Jun Du.

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The GenBank accession numbers for the 16S rRNA gene sequence of Lujinxingia vulgaris TMQ4Tand TMQ2 are MH613067 and MN547342, respectively. The draft genome of Lujinxingia vulgaris TMQ4T and TMQ2 has been deposited in GenBank under the Accession Numbers VOSM00000000 and VOSL00000000, separately.

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Wang, S., Mu, DS., Li, GY. et al. Description of Lujinxingia vulgaris sp. nov., isolated from coastal sediment via prey-traps. Antonie van Leeuwenhoek 114, 1805–1818 (2021). https://doi.org/10.1007/s10482-021-01640-5

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  • DOI: https://doi.org/10.1007/s10482-021-01640-5

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