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Pseudooceanicola albus sp. nov., Isolated from Mangrove Sediment within the Beibu Gulf

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Abstract

A nonmotile gram-negative, short, rod-shaped strain, BGMRC 2024T, was isolated from the sediment of Rhizophora stylosa collected in the Beibu Gulf, China. The strain BGMRC 2024T exhibited optimal growth at 25 to 28°C, pH 7.0, and in the presence of 1–4% (wt/v) NaCl. Analysis of the 16S rRNA gene sequences indicated that BGMRC 2024T was closely related to Pseudooceanicola antarcticus CGMCC 1.12662T (96.23% sequence similarity), P. algae Lw-13eT (96.17%), P. lipolyticus 157T (96.16%), and P. pacificus 216_PA32_1T (96.09%). The digital DNA-DNA hybridization (dDDH) values and the average nucleotide identity (ANI) of the strain Pseudooceanicola antarcticus CGMCC 1.12662T were 24.7 and 77.52%, respectively. The DNA G+C content was 67.4 mol %. The main respiratory quinone was ubiquinone-10. The polar lipids of strain BGMRC 2024T included four unidentified phospholipids, two unidentified ninhydrin-positive lipids, one unidentified ninhydrin-positive phospholipid, and three unidentified lipids. The major fatty acids were C19:0 cyclo ω8c, summed feature 8 (C18:1ω7c and/or C18:1ω6c), and C16:0. Based on these results, BGMRC 2024T represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicola albus sp. nov. is proposed. The type strain is BGMRC 2024T (=KCTC52117T = DSM102086T).

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Funding

The work was supported by the Joint Fund Project for Regional Innovation and Development set up by the National Natural Science Foundation (U20A20101), the Natural Science Foundation of Guangxi (2020GXNS-FGA297002), Middle-Aged Teachers Scientific Research Project of Guangxi Universities (2022KY0283), Youth Fund Project of Guangxi University of Chinese Medicine (2021QN009), Special Fund for Bagui Scholars of Guangxi and the Innovation Project of Guangxi Graduate Education (YCSW2022356), Special Fund for Bagui Scholars of Guangxi, High-level Talent Inheritance and Innovation Team of Guangxi Traditional Chinese Medicine (2022A007), Guangxi University of Chinese Medicine “Guipai Traditional Chinese Medicine Inheritance and Innovation Team” Project (2022B005), Guangxi University of Chinese Medicine-Guipai Xinglin Top Talent Funding Project (2022C008).

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Authors and Affiliations

  1. Institute of Marine Drugs, Guangxi University of Chinese Medicine, 530200, Nanning, China

    M. Li, F. T. Li, C. H. Gao, Y. H. Liu & X. X. Yi

  2. Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, 530200, Nanning, China

    M. Li, F. T. Li, C. H. Gao, Y. H. Liu & X. X. Yi

  3. School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, 530200, Nanning, China

    F. T. Li & X. X. Yi

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Li, M., Li, F.T., Gao, C.H. et al. Pseudooceanicola albus sp. nov., Isolated from Mangrove Sediment within the Beibu Gulf. Microbiology 92, 622–629 (2023). https://doi.org/10.1134/S0026261723601070

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