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Lactobacillus gastricus BTM 7 prevents intestinal colonization by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model host

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Abstract

The study reports protective role of potential probiotic cultures against infection by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model system. Among the fifteen indigenous potential probiotics, the cell free supernatant of Lactobacillus gastricus BTM7 possessed highest antimicrobial action and biofilm inhibition against C. sakazakii. The competitive exclusion assays revealed that preconditioning with probiotics resulted in increased mean life span of the nematode to 12–13 days as compared to 5–6 days when the pathogen was administered alone. Enhanced expression of the marker genes (pmk-1, daf-16 and skn-1) was observed during the administration of probiotic cultures. The highest expression of pmk-1 (2.5 folds) was observed with administration of L. gastricus BTM7. The principal component analysis on selected variables revealed that L. gastricus BTM7 has the potential to limit the infection of C. sakazakii in C. elegans and enhance the expression of key genes involved in extending life span of the worm.

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Acknowledgements

The authors wish to thank Department of Biotechnology (DBT), Govt. of India, for providing financial support and Jaypee University of Information Technology for essential facilities required for the research work.

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Authors and Affiliations

  1. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, 173234, India

    Sharma Kavita & Gunjan Goel

  2. Department of Biotechnology, Alagappa University, Karaikudi, 630004, India

    Murugesan Pooranachithra, Mani Iyer Prasanth & Krishnaswamy Balamurugan

  3. Department of Biotechnology, Faculty of Engineering and Technology, Rama University, Mandhana, Kanpur, 209217, India

    Niharika Singh

  4. Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand

    Mani Iyer Prasanth

  5. Department of Microbiology, School of Interdisciplinary and Applied Sciences, Central University of Haryana, Mahendergarh, 123031, India

    Gunjan Goel

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  1. Sharma Kavita
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  2. Murugesan Pooranachithra
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  3. Niharika Singh
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  4. Mani Iyer Prasanth
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  5. Krishnaswamy Balamurugan
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  6. Gunjan Goel
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Contributions

KS, PM, MIP performed and executed the experiments, KS,NS, KB and GG contributed to text preparation and revised the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Gunjan Goel.

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Kavita, S., Pooranachithra, M., Singh, N. et al. Lactobacillus gastricus BTM 7 prevents intestinal colonization by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model host. Antonie van Leeuwenhoek 113, 1587–1600 (2020). https://doi.org/10.1007/s10482-020-01466-7

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