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Blastococcus colisei sp. nov, isolated from an archaeological amphitheatre

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Abstract

The taxonomic position of an actinobacterial isolate, designated strain BMG 822T, isolated from limestone from the Amphitheater of El Jem (Coliseum Thysdrus), Tunisia, was established using a polyphasic approach. Strain BMG 822T was found to grow well at 30 °C and pH 6.5–8.0, and to be coral-coloured, Gram-positive, catalase and oxidase negative. Whole cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, glucose, galactose and ribose. The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified glycophospholipid and six unidentified phospholipids. MK-9(H4) was found to be the predominant menaquinone, followed by MK-9(H2) and MK-9. The major cellular fatty acids were identified as iso-C16:0, C18:1 ω9c, C17:1 ω8c and iso-H-C16:1. The G+C content of the DNA (73.2%) is typical of the genus. High degrees of 16S rRNA gene sequence similarity were found with the type strains of the genus Blastococcus (97.1–98.3%) followed by the type strains of Modestobacter (96.8–97.8%). Based on the above data and the phenotypic differences from the type strains of Blastococcus species, it is proposed that the isolate BMG 822T (=DSM 46837T=CECT 8823T) should be classified as the type strain of a novel species, Blastococcus colisei sp. nov.

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Acknowledgements

This research was supported by The Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, Tunisia (LR03ES03).

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Authors and Affiliations

  1. Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia

    Karima Hezbri, Maher Gtari & Faten Ghodhbane-Gtari

  2. School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK

    Imen Nouioui, Hans-Peter Klenk & Maria del Carmen Montero-Calasanz

  3. Central Facility for Microscopy, HZI – Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany

    Manfred Rohde

  4. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany

    Peter Schumann & Maria del Carmen Montero-Calasanz

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  1. Karima Hezbri
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  2. Imen Nouioui
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  3. Manfred Rohde
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  4. Peter Schumann
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  5. Maher Gtari
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  7. Maria del Carmen Montero-Calasanz
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Correspondence to Maria del Carmen Montero-Calasanz or Faten Ghodhbane-Gtari.

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Hezbri, K., Nouioui, I., Rohde, M. et al. Blastococcus colisei sp. nov, isolated from an archaeological amphitheatre. Antonie van Leeuwenhoek 110, 339–346 (2017). https://doi.org/10.1007/s10482-016-0804-4

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