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Description of Geodermatophilus bullaregiensis sp. nov.

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Abstract

The taxonomic position of an aerobic actinobacterial strain, BMG841T, isolated from the Bulla Regia monument (Tunisia) and exhibiting a high resistance to gamma-radiation (D10 ~9 kGy) was determined using polyphasic approach. The optimal growth range was found to be 25–35 °C at pH of 7.0–8.5. The strain was observed to form black dry colonies. Chemotaxonomic characteristics of the isolate showed a cell wall type III, with galactose and glucose as diagnostic sugars; phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid as main polar lipids; and MK-9(H4) as the predominant menaquinone. The major cellular fatty acids were identified as iso-C16:0 and iso-C15:0. Phylogenetic analysis indicated that strain BMG841T represents a novel member of the genus Geodermatophilus with high 16S rRNA gene sequence identity with Geodermatophilus saharensis (98.28 %). Based on phylogenetic and phenotypic analysis, strain BMG841T is proposed as the type strain (=DSM 46841T = CECT 8821T) of a novel species, Geodermatophilus bullaregiensis.

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Acknowledgments

This research was supported by The Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, Tunisia (LR03ES03). Chemotaxonomic work was done at the DSMZ (German Collection of Microorganisms and Cell Cultures) Braunschweig. The authors would like to thank Professor Bernhard Schink for his advice in naming the new species.

Conflict of interest

Authors disclose that there are no conflicts of interest. No research involving human participants and/or animals was performed. No financial interests tied directly or indirectly to this research exist that may be important to readers need to be disclosed.

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Authors and Affiliations

  1. Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia

    Karima Hezbri, Faten Ghodhbane-Gtari & Maher Gtari

  2. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany

    Maria del Carmen Montero-Calasanz, Peter Schumann & Hans-Peter Klenk

  3. School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK

    Maria del Carmen Montero-Calasanz & Hans-Peter Klenk

  4. Centre National des Sciences et Technologies Nucléaires, BP 72, Pôle Technologique, 2020, Sidi Thabet, Tunisia

    Haïtham Sghaier

  5. Central Facility for Microscopy, HZI – Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany

    Manfred Rohde

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  1. Karima Hezbri
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  2. Faten Ghodhbane-Gtari
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Corresponding author

Correspondence to Maher Gtari.

Electronic supplementary material

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Supplementary material 1 (PPT 205 kb)

Supplementary Fig. S1. The parameter “Maximum Height” based on respiration curves generated with the OmniLog phenotyping illustrated as a heatmap using the opm package. Plates and substrates are rearranged according to their overall similarity (as depicted using the row and column dendrograms). Ochre colour indicates positive reaction; purple colour indicates negative reaction; white colour indicates ambiguous reaction. Letters (A/B) correspond to the two experimental replicates

Supplementary material 2 (PPT 314 kb)

Supplementary Fig. S2. Two-dimensional TLC polar lipids profiling of strain BMG841T after revelation with molydatophosphoric acid spraying. DPG, diphosphadidylglycerol; PE, phosphatidethanolamine; PC, phosphatidylcholine; PI, phosphatidylinositol; GL, unidentified glycolipid

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Hezbri, K., Ghodhbane-Gtari, F., del Carmen Montero-Calasanz, M. et al. Description of Geodermatophilus bullaregiensis sp. nov.. Antonie van Leeuwenhoek 108, 415–425 (2015). https://doi.org/10.1007/s10482-015-0494-3

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  • DOI: https://doi.org/10.1007/s10482-015-0494-3

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