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Savagea faecisuis gen. nov., sp. nov., a tylosin- and tetracycline-resistant bacterium isolated from a swine-manure storage pit

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Abstract

A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular methods was performed on three strains of a Gram-stain positive, non-sporeforming, motile aerobic rod-shaped bacterium resistant to tylosin and tetracycline isolated from a swine-manure storage pit. On the basis of 16S rRNA gene sequence analyses, it was confirmed that these isolates are highly related to each other and form a hitherto unknown lineage within the Planococcaceae. In particular, pairwise analysis of the 16S rRNA gene sequence demonstrated that the novel organism is closely related to members of the genus Sporosarcina (92.8–94.5 %), Pyschrobacillus (93.5–93.9 %) and Paenisporosarcina (93.3–94.5 %). The predominant fatty acids were found to consist of iso-C15:0 and iso-C17:1 ω10c and the G+C mol% was determined to be 41.8. Based on biochemical, chemotaxonomic, and phylogenetic evidence, it is proposed that these novel strains be classified as a novel genus and species, Savagea faecisuis gen nov., sp. nov. The type strain is Con12T (=CCUG 63563T = NRRL B-59945T = NBRC 109956T).

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Acknowledgments

The authors also wish to acknowledge the valuable technical assistance by Rhonda Zeltwanger. We also thank Ken-ichiro Suzuki at the Biological Resource Center, National Institute of Technology and Evaluation (NBRC) for facilitating the analysis of the peptidoglycan. Thanks are also given to Jürgen Busse, Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Austria, for the quinone analysis. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual’s income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA’s TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer.

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    Authors and Affiliations

    1. Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA

      Terence R. Whitehead & Michael A. Cotta

    2. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, 73019, USA

      Crystal N. Johnson, Nisha B. Patel & Paul A. Lawson

    3. Culture Collection, Department of Clinical Bacteriology, University of Göteborg, 41346, Göteborg, Sweden

      Edward R. B. Moore

    4. Ecology and Evolutionary Biology Program, University of Oklahoma, Norman, OK, 73019, USA

      Paul A. Lawson

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    1. Terence R. Whitehead
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    Correspondence to Terence R. Whitehead or Paul A. Lawson.

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    Terence R. Whitehead and Paul A. Lawson have equally contributed to the work.

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    Whitehead, T.R., Johnson, C.N., Patel, N.B. et al. Savagea faecisuis gen. nov., sp. nov., a tylosin- and tetracycline-resistant bacterium isolated from a swine-manure storage pit. Antonie van Leeuwenhoek 108, 151–161 (2015). https://doi.org/10.1007/s10482-015-0473-8

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