Skip to main content
SpringerLink
Log in

Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

An Erratum to this article was published on 17 April 2013

An Erratum to this article was published on 16 November 2012

Abstract

A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular genetic methods was performed on six strains of unknown Gram-positive, nonspore-forming, facultative anaerobic coccus-shaped bacteria isolated from a swine-manure storage pit. On the basis of the 16S rRNA, RNA polymerase α-subunit (rpoA) and 60 kDa chaperonin (cpn60) gene sequence analyses, it was shown that all the isolates were enterococci but formed two separate lines of descent. Pairwise 16S rRNA gene sequence comparisons demonstrated that the two novel organisms were most closely related to each other (97.9 %) and to Enterococcus aquimarinus (97.8 %). Both organisms contained major amounts of C16:0, C16:1 ω7c, C16:1 ω7c, and C18:1 ω7c/12t/9t as the major cellular fatty acids. Based on biochemical, chemotaxonomic and phylogenetic evidence, the names Enterococcus lemanii sp. nov. (type strain PC32T = CCUG 61260T = NRRL B-59661T) and Enterococcus eurekensis sp. nov. (type strain PC4BT = CCUG 61259T = NRRL B-59662T) are proposed for these hitherto undescribed species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Andrighetto C, De Dea P, Lombardi A, Neviani E, Rossetti L, Giraffa G (1998) Molecular identification and cluster analysis of homofermentative thermophilic lactobacilli isolated from dairy products. Res Microbiol 149:631–643

    Article  PubMed  CAS  Google Scholar 

  • Bryant MP (1972) Interactions among intestinal microorganisms. Am J Clin Nutr 25:1485–1487

    PubMed  CAS  Google Scholar 

  • Cotta MA, Whitehead TR, Zeltwanger RL (2003) Isolation, characterization and comparison of bacteria from swine faeces and manure storage pits. Environ Microbiol 5:737–745

    Article  PubMed  CAS  Google Scholar 

  • Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PA (2009) Robinsoniella peoriensis gen. nov., sp. nov., isolated from a swine-manure storage pit and a human clinical source. Int J Syst Evol Microbiol 55:150–155

    Article  Google Scholar 

  • Dehority BA, Grubb JA (1976) Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources. Appl Environ Microbiol 32:703–710

    PubMed  CAS  Google Scholar 

  • Descheemaeker P, Lammens C, Pot B, Vandamme P, Goossens H (1997) Evaluation of arbitrarily primed PCR analysis and pulse-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine. Int J Syst Bacteriol 47:555–561

    Article  PubMed  CAS  Google Scholar 

  • Feilberg A, Dezhao L, Adamsen A, Hansen M, Jonassen K (2010) Odorant emissions from intensive pig production measured by online proton-transfer-reaction mass spectrometry. Environ Sci Technol 44:5894–5900

    Article  PubMed  CAS  Google Scholar 

  • Harwood VJ, Whitlock J, Withington V (2000) Classification of antibiotic resistance patterns of indicator bacteria by discriminant analysis: use in predicting the source of fecal contamination in subtropical waters. Appl Environ Microbiol 66:3698–3704

    Article  PubMed  CAS  Google Scholar 

  • Hespell RB, Wolf R, Bothast RJ (1987) Fermentation of xylans by Butyrivibrio fibrisolvens and other ruminal bacteria. Appl Environ Microbiol 53:2849–2853

    PubMed  CAS  Google Scholar 

  • Johnson JL (1994) Similarity analysis of DNAs. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. ASM, Washington, DC, pp 656–682

    Google Scholar 

  • Le P, Aarnink A, Ogink N, Becker P, Verstegen M (2005) Odour from animal production facilities: its relationship to diet. Nutr Res Rev 18:3–30

    Article  PubMed  Google Scholar 

  • Leedle JA, Hespell RB (1980) Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations. Appl Environ Microbiol 39:709–719

    PubMed  CAS  Google Scholar 

  • Marks R (2001) Cesspools of shame: how factory farm lagoons and sprayfields threaten environmental and public health. Report from NRDC and the Clean Water Network. http://www.nrdc.org/water/pollution/cesspools/cessinx.asp

  • Miescier JJ, Cabelli VJ (1982) Enterococci and other microbial indicators in municipal wastewater effluents. J Water Pollut Control Fed 54:1599–1606

    Google Scholar 

  • Miller LT (1982) Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586

    PubMed  CAS  Google Scholar 

  • Miller DN (2001) Accumulation and consumption of odorous compounds in feedlot soils under aerobic, fermentative, and anaerobic respiratory conditions. J Anim Sci 79:2503–2512

    PubMed  CAS  Google Scholar 

  • Naser SM, Thompson FL, Hoste B, Gevers D, Dawyndt P, Vancanneyt M, Swings J (2005) Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150

    Article  PubMed  CAS  Google Scholar 

  • Nicholas KB, Nicholas HB Jr, Deerfield DW II (1997) GeneDoc: analysis and visualization of genetic variation. EMBNEW News 4:14

    Google Scholar 

  • Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  • Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448

    Article  PubMed  CAS  Google Scholar 

  • Rasmussen SW (2002) SEQtools, a software package for analysis of nucleotide and protein sequences. http://www.seqtools.dk. Accessed 3 Aug 2012

  • Saito H, Miura K-I (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophysica Acta 72:619–629

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, in: MIDI technical note 101. MIDI, Inc., Newark

    Google Scholar 

  • Sistek V, Maheux AF, Boissinot M, Bernard KA, Cantin P, Cleenwerck I, de Vos P, Bergeron MG (2011) Two novel species, Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.029033-0

  • Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155

    Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Švec P, Devriese LA (2009) Genus I. Enterococcus. In: de Vos P, Garrity GM, Jones D, Kreig NR, Rainey FA, Schleifer K-H, Whitman WB (eds) Bergyey’s manual of systematic bacteriology, vol 3, 2nd edn. The Firmicutes, Springer, New York, pp 594–607

    Google Scholar 

  • Švec P, Vancanneyt M, Devriese LA, Naser SM, Snauwaert C, Lefebvre K, Hoste B, Swings J (2005) Enterococcus aquimarinus sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55:2183–2187

    Article  PubMed  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Clustalw: improving the sensitivity for progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Tindall BJ, Sikorski J, Smibert RM, Kreig NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology. ASM Press, Washington, DC, pp 330–393

    Google Scholar 

  • Vermette C, Russell A, Desai A, Hill J (2010) Resolution of phenotypically distinct strains of Enterococcus spp. in a complex microbial community using cpn60 universal target sequencing. Microb Ecol 59:14–24

    Article  PubMed  CAS  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Triiper HG (1987) Report of the Ad Hoc Committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Weiss A, Domig KJ, Kneifel W (2005) Comparison of selective media for the enumeration of probiotic enterococci from animal feed. Food Technol Biotechnol 43:147–155

    Google Scholar 

  • Whitehead TR, Cotta MA (2001) Characterization of microbial populations in swine feces and waste storage pits by 16S rDNA gene sequence analysis. Anaerobe 7:181–187

    Article  CAS  Google Scholar 

  • Whitehead TR, Cotta MA (2004) Isolation and identification of hyper-ammonia producing bacteria from swine manure storage pits. Curr Microbiol 48:20–26

    Article  PubMed  CAS  Google Scholar 

  • Whitehead TR, Flint HJ (1995) Heterologous expression of an endoglucanase gene (endA) from the ruminai anaerobe Ruminococcus flavefaciens 17 in Streptococcus bovis and Streptococcus sanguis. FEMS Microbiol Lett 126:165–169

    Article  PubMed  CAS  Google Scholar 

  • Whitehead TR, Cotta MA, Collins MD, Lawson PA (2003) Description of Hespellia stercorisuis gen. nov., sp. nov., and Hespellia porcinus sp. nov., isolated from manure storage pits. Int J Syst Evol Microbiol 54:241–245

    Article  Google Scholar 

  • Whitehead TR, Cotta MA, Collins MD, Falsen E, Lawson PA (2005) Bacteroides coprosuis sp. nov., isolated from swine-manure storage pits. Int J Syst Evol Microbiol 55:2515–2518

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to acknowledge Janet Hill, Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Canada for assistance with the analysis of the cpn60 gene sequences. The authors also wish to acknowledge the valuable technical assistance by Rhonda Zeltwanger.

Author information

Authors and Affiliations

  1. Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA

    Michael A. Cotta & Terence R. Whitehead

  2. Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-41346, Göteborg, Sweden

    Enevold Falsen & Edward Moore

  3. Department of Botany and Microbiology, University of Oklahoma, Norman, OK, 73019, USA

    Paul A. Lawson

  4. Ecology and Evolutionary Biology Program, University of Oklahoma, Norman, OK, 73019, USA

    Paul A. Lawson

Authors
  1. Michael A. Cotta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Terence R. Whitehead
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Enevold Falsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Edward Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paul A. Lawson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Terence R. Whitehead.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 412 kb)

Supplementary material 2 (DOCX 508 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cotta, M.A., Whitehead, T.R., Falsen, E. et al. Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit. Antonie van Leeuwenhoek 103, 89–98 (2013). https://doi.org/10.1007/s10482-012-9789-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-012-9789-9

Keywords

Search

Navigation