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Lysinibacillus fluoroglycofenilyticus sp. nov., a bacterium isolated from fluoroglycofen contaminated soil

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Abstract

A novel Gram-positive, fluoroglycofen-degrading bacterium, designated cmg86T, was isolated from herbicide contaminated soil collected from Tongjing, Jiangsu province, China. Strain cmg86T was found to be aerobic, motile, endospore-forming rods. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain cmg86T belongs to the genus Lysinibacillus and showed the highest sequence similarity to Lysinibacillus meyeri DSM 25057T (97.9 %) and Lysinibacillus odysseyi KCTC 3961T (96.6 %). The cell-wall peptidoglycan type was determined to be A4α (l-Lys-d-Asp), which is consistent with the cell-wall characteristics of the genus Lysinibacillus. The predominant respiratory quinones were identified as menaquinone-7 (MK-7, 89.5 %) and meanaquinone-6 (MK-6, 8.9 %), and the major fatty acids were identified as iso-C15:0, anteiso-C15:0 and antesio-C17:0. The major polar lipids were found to be phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content of strain cmg86T was determined to be 37.6 mol%. The results of this study support the conclusion that strain cmg86T represents a novel species of the genus Lysinibacillus for which the name and Lysinibacillus fluoroglycofenilyticus sp. nov. is proposed. The type strain is cmg86T (=KCTC 33183T = CCTCC AB 2013247T).

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Acknowledgment

This work was supported by the National High Technology Research and Development Program of China (2012AA101403, 2013AA102804), the Social Development Program Fund of Jiangsu Province (BE2011783).

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Authors and Affiliations

  1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Minggen Cheng, Hao Zhang, Jing Zhang, Jian He & Xing Huang

  2. Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Gang Hu

  3. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Jun Zhang

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  1. Minggen Cheng
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  2. Hao Zhang
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  3. Jing Zhang
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  4. Gang Hu
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  5. Jun Zhang
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  6. Jian He
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  7. Xing Huang
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Correspondence to Xing Huang.

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Cheng, M., Zhang, H., Zhang, J. et al. Lysinibacillus fluoroglycofenilyticus sp. nov., a bacterium isolated from fluoroglycofen contaminated soil. Antonie van Leeuwenhoek 107, 157–164 (2015). https://doi.org/10.1007/s10482-014-0313-2

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  • DOI: https://doi.org/10.1007/s10482-014-0313-2

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