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Taishania pollutisoli gen. nov., sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil

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Abstract

Strain CZZ-1T was isolated from long-term TBBPA-contaminated soil Zaozhuang city, Shandong province, People’s Republic of China. CZZ-1T was pink-pigmented, Gram-stain-negative, rod-shaped, non-motile and aerobic. The 16S rRNA gene analysis indicated that strain CZZ-1T shows high similarities to Fluviicola taffensis DSM 16823T (92.6%) and Fluviicola hefeinensis KACC 16597T (92.5%) and less than 91% sequence similarities to other genus or species in the family Crocinitomicaceae. It was able to grow at 10–37 °C, with 0–6% (w/v) NaCl. It could hydrolyze gelatin, but could not reduce nitrates to nitrites. The predominant fatty acids of strain CZZ-1T were iso-C15:0 (51.3%), C15:0 2-OH (11.0%), iso-C17:0 3-OH (8.0%), C14: 0 (7.0%), iso-C15:1 G (6.8%) and Summed Feature 3 (C16:1 ω7c and/or C16:1 ω6c, 4.4%). The polar lipid profile was composed of five unidentified lipids, two unidentified phospholipids, one phosphatidylethanolamine, one unidentified aminolipid and one unidentified glycolipid. The predominant respiratory quinone was MK-6. The genomic DNA G+C content of strain CZZ-1T was 41.5 mol%. Based on data from phenotypic, chemotaxonomic and genotypic analysis in this study, strain CZZ-1T represents a novel species in a new genus in the family Crocinitomicaceae, for which the name Taishania pollutisoli gen. nov., sp. nov. is proposed. The type strain is CZZ-1T (= KCTC 52343T = GDMCC 1.2270T).

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Acknowledgements

This work was funded by grants from Shandong Provincial University Youth Innovation and Technology Program, China (2020KJE008 and 2019KJE020) and the National natural science foundation of China (31600080); the Science and Technology Development Program of Zaozhuang (2020NS09).

Funding

This work was funded by grants from Shandong Provincial University Youth Innovation and Technology Program, China (2020KJE008 and 2019KJE020) and the National natural science foundation of China (31600080); the Science and Technology Development Program of Zaozhuang (2020NS09).

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Authors and Affiliations

  1. Key Lab of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Minjian Lan, Tunan Yang & Kai Chen

  2. Institute of Organic Contaminant Control and Soil Remediation, College of Resource and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Qing Chen

  3. College of Life Sciences, Zaozhuang University, 277160, Zaozhuang, People’s Republic of China

    Yue Song, Lijie Lv, Hongmei Wang & Qing Chen

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  1. Minjian Lan
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Contributions

Material preparation, data collection and analysis were performed by ML and TY, Material preparation and data collection were performed by LL; data collection and analysis were performed by HW. QC contributed to original draft preparation ; QC and KC contributed to review and editing. All authors read and approved the final manuscript.

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Correspondence to Qing Chen or Kai Chen.

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Lan, M., Yang, T., Song, Y. et al. Taishania pollutisoli gen. nov., sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil. Curr Microbiol 78, 2563–2568 (2021). https://doi.org/10.1007/s00284-021-02522-9

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