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Physiology and gene expression profiles of Dekkera bruxellensis in response to carbon and nitrogen availability

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Abstract

The assimilation of nitrate, a nitrogenous compound, was previously described as an important factor favoring Dekkera bruxellensis in the competition with Saccharomyces cerevisiae for the industrial sugarcane substrate. In this substrate, nitrogen sources are limited and diverse, and a recent report showed that amino acids enable D. bruxellensis to grow anaerobically. Thus, understanding the regulation of nitrogen metabolism is one fundamental aspect to comprehend the competiveness of D. bruxellensis in the fermentation environment. In the present study, we evaluated the physiological and transcriptional profiles of D. bruxellensis in response to different carbon and nitrogen supplies to determine their influence on growth, sugar consumption, and ethanol production. Besides, the expression of genes coding for nitrogen permeases and enzymes involved in the biosynthesis of glutamate and energetic metabolism were investigated under these conditions. Our data revealed that genes related to nitrogen uptake in D. bruxellensis are under the control of nitrogen catabolite repression. Moreover, we provide indications that glutamate dehydrogenase and glutamate synthase may switch roles as the major pathway for glutamate biosynthesis in D. bruxellensis. Finally, our data showed that in nonoptimal growth conditions, D. bruxellensis leans toward the respiratory metabolism. The results presented herein show that D. bruxellensis and S. cerevisiae share similar regulation of GDH–GOGAT pathway, while D. bruxellensis converts less glucose to ethanol than S. cerevisiae do when nitrogen is limited. The consequence of this particularity to the industrial process is discussed.

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Acknowledgments

This study was sponsored by the Bioethanol Research Network of the State of Pernambuco (CNPq-FACEPE/PRONEM program) and the Brazilian funding agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE). VP was supported by the MicroDrive-programme at the NL-faculty of SLU.

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Authors and Affiliations

  1. Interdepartmental Research Group on Metabolic Engineering, Federal University of Pernambuco, Av. Moraes Rego 1235, Recife, PE, 50670-901, Brazil

    Will de Barros Pita, Denise Castro Silva, Diogo Ardaillon Simões & Marcos Antonio de Morais Jr.

  2. Department of Biochemistry, Federal University of Pernambuco, Av. Moraes Rego 1235, Recife, PE, 50670-901, Brazil

    Diogo Ardaillon Simões

  3. Department of Genetics, Federal University of Pernambuco, Av. Moraes Rego, 1235 Cidade Universitária, Recife, PE, 50670-901, Brazil

    Will de Barros Pita & Marcos Antonio de Morais Jr.

  4. Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Volkmar Passoth

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  1. Will de Barros Pita
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  5. Marcos Antonio de Morais Jr.
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Correspondence to Will de Barros Pita.

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de Barros Pita, W., Silva, D.C., Simões, D.A. et al. Physiology and gene expression profiles of Dekkera bruxellensis in response to carbon and nitrogen availability. Antonie van Leeuwenhoek 104, 855–868 (2013). https://doi.org/10.1007/s10482-013-9998-x

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