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Parvularcula oceanus sp. nov., isolated from deep-sea water of the Southeastern Pacific Ocean

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An Erratum to this article was published on 19 December 2013

Abstract

A Gram-negative, aerobic, motile by single polar flagellum, short rod-shaped bacterium was isolated from a deep-seawater sample of the Southeastern Pacific Ocean. Growth was found to occur at 10–40 °C, at pH 4.0–10.0 and in the presence of 0–9.0 % (w/v) NaCl. The phylogenetic tree based on 16S rRNA gene sequences showed that strain JLT2013T fell within a clade comprising species of the genus Parvularcula and formed a coherent cluster with Parvularcula lutaonensis CC-MMS-1T (neighbour-joining phylogenetic tree) or Parvularcula dongshanensis SH25T (maximum-likelihood and maximum-parsimony phylogenetic trees). Sequence similarity analyses based on 16S rRNA gene sequences revealed that strain JLT2013T shows high sequence similarity to P. lutaonensis CC-MMS-1T (96.7 %), P. dongshanensis SH25T (96.0 %) and Parvularcula bermudensis HTCC2503T (95.2 %). The major cellular fatty acids were identified as C12:0 (34.3 %), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) (10.9 %), C16:0 (10.0 %) and C17:1 ω6c (7.2 %). The polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol, three sphingoglycolipids and three unknown glycolipids. Strain JLT2013T was found to contain Q-10 as the predominant quinone. The DNA G+C content was determined to be 66.3 mol%. In the light of the phenotypic characteristics, chemotaxonomic features and phylogenetic evidence gathered in this study, strain JLT2013T (=LMG 27362T = CGMCC 1.12400T) should be classified as a novel species in the genus Parvularcula, for which the name Parvularcula oceanus sp. nov. is proposed.

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Acknowledgements

This work was supported by the National Key Basic Research Program of China (2013CB955700), the SOA Project (201105021), the National Natural Science Foundation of China project (41276131, 91028001) and MELRI1304.

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Authors and Affiliations

  1. State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen, 361005, People’s Republic of China

    Shuhui Li, Kai Tang, Keshao Liu & Nianzhi Jiao

  2. Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, People’s Republic of China

    Chang-Ping Yu

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  1. Shuhui Li
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  2. Kai Tang
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  3. Keshao Liu
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  4. Chang-Ping Yu
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  5. Nianzhi Jiao
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Correspondence to Kai Tang.

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Li, S., Tang, K., Liu, K. et al. Parvularcula oceanus sp. nov., isolated from deep-sea water of the Southeastern Pacific Ocean. Antonie van Leeuwenhoek 105, 245–251 (2014). https://doi.org/10.1007/s10482-013-0071-6

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