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Diversity of fungi in creosote-treated crosstie wastes and their resistance to polycyclic aromatic hydrocarbons

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Abstract

This study was conducted to generate information regarding the diversity of fungi inhabiting creosote-treated wood in a storage yard for crosstie wastes in Gwangmyeong, Korea. Additionally, the resistance to polycyclic aromatic hydrocarbons (PAHs) of indigenous fungi that mainly occupy creosote-treated wood was evaluated. We isolated fungi from the surface and inner area of crosstie wastes and identified them using a combination of traditional methods and molecular techniques. Overall, 179 isolates including 47 different species were isolated from 240 sampling sites. The identified fungal species included 23 ascomycetes, 19 basidiomycetes, and 5 zygomycetes. Three species, Alternaria alternata, Irpex lacteus, and Rhizomucor variabilis, were the most frequently isolated ascomycetes, basidiomycetes, and zygomycetes, respectively. The results of this study showed that there was a large difference in the fungal diversity between the surface and the inner area. Additionally, zygomycetes and ascomycetes were found to have a greater tolerance to PAHs than basidiomycetes. However, two basidiomycetes, Heterobasidion annosum and Schizophyllum commune, showed very high resistance to PAHs, even in response to the highest concentration (1,000 ppm), which indicates that these species may play a role in the degradation of PAHs.

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Acknowledgments

This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (KRF-2008-331-F00029) and by Technology Development Program (309016-5) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, Korea

    Min-Ji Kim, Hwanhwi Lee, Yong-Seok Choi, Gyu-Hyeok Kim, Na-Yoon Huh, Sangjoon Lee & Jae-Jin Kim

  2. National Institute of Biological Resources, Incheon, Korea

    Young Woon Lim

  3. Division of Forest Bioenergy, Korea Forest Research Institute (KFRI), 57 Hoegiro, Dongdaemun-gu, Seoul, 130-712, Korea

    Sung-Suk Lee

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  1. Min-Ji Kim
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Kim, MJ., Lee, H., Choi, YS. et al. Diversity of fungi in creosote-treated crosstie wastes and their resistance to polycyclic aromatic hydrocarbons. Antonie van Leeuwenhoek 97, 377–387 (2010). https://doi.org/10.1007/s10482-010-9416-6

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