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In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes

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Abstract

Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 μg ml−1 when it was used alone, or at constant concentration (100 μg ml−1) in combination with FCZ at from 0.25 to 32 μg ml−1. The MICs for FCZ were found to be between 0.25 and 128 μg ml−1. PAF caused a fungicidal effect at 200 μg ml−1 and reduced growth at between 50 and 200 μg ml−1. Total growth inhibition with fungistatic activity was detected at 64 μg ml−1 of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 μg ml−1 FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.

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Abbreviations

ADD:

Additive effect

FCZ:

Fluconazole

FI:

Full inhibition

IR:

Interaction ratio

NI:

No interaction

PAF:

Penicillium chrysogenum antifungal protein

SY:

Synergism

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Acknowledgements

I.P. was supported by GENOMNANOTECH-DEBRET (RET-06/2004). The financial support received from the Öveges József Program of the Hungarian National Office for Research and Technology (grant reference numbers OMFB 01501/2006 and 01528/2006) is also gratefully acknowledged. T.P. holds a János Bolyai Research Scholarship from the Hungarian Academy of Sciences. This research was also supported in part by ETT 214/2006.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52., 6726, Szeged, Hungary

    László Galgóczy, Tamás Papp & Csaba Vágvölgyi

  2. Department of Microbiology and Biotechnology, Faculty of Sciences, University of Debrecen, P.O. Box 63, 4010, Debrecen, Hungary

    István Pócsi & Nikoletta Hegedűs

Authors
  1. László Galgóczy
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  2. Tamás Papp
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  3. István Pócsi
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  4. Nikoletta Hegedűs
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  5. Csaba Vágvölgyi
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Correspondence to László Galgóczy.

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Galgóczy, L., Papp, T., Pócsi, I. et al. In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes. Antonie van Leeuwenhoek 94, 463–470 (2008). https://doi.org/10.1007/s10482-008-9263-x

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  • DOI: https://doi.org/10.1007/s10482-008-9263-x

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