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In vitro antifungal activity of heterocyclic organoboron compounds against Trichophyton mentagrophytes and Microsporum canis obtained from clinical isolates

  • Clinical Microbiology - Research Paper
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Abstract  

The aim of this study was to investigate the in vitro activity of thirty-eight heterocyclic organoboron compounds (1a–o, 2a–j, 3a–m) against clinically isolated dermatophytes Trichophyton mentagrophytes and Microsporum canis. Minimum inhibitory concentrations (MICs) of compounds (1a–o, 2a–j, 3a–m) were determined according to published protocol Clinical and Laboratory Standards Institute (CLSI) M38–A2 broth microdilution method. The minimum fungicidal concentrations (MFCs) for both T. mentagrophytes and M. canis were found by subculturing each fungal suspension on potato dextrose agar. According to the results, heterocyclic organoboron compounds (1a–o, 2a–j, 3a–m) were found to be more effective against dermatophyte M. canis (MIC = 3.12–25 µg/ml) than T. mentagrophytes (MIC = 12.5–100 µg/ml). Our findings showed that 7–membered heterocyclic organoboron compounds (3a–m) (MIC = 12.5–50 µg/ml) have stronger in vitro antifungal activity against T. mentagrophytes than 5–membered heterocyclic organoboron compounds (1a–o, 2a–j) (MIC = 25–100 µg/ml). The MFC values for all compounds ranged from 6.25 to 200 µg/ml. The limited number of systemic antifungal agents used in the treatment of dermatophyte infections and the presence of side effects have led to the search for new treatment resources in recent years. Therefore, investigation of the effect of heterocyclic organoboron compounds against dermatophytes will be promising for the discovery of new antifungal compounds that have gained great importance today.

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Funding

This work was supported by Kocaeli University Scientific Research Projects Coordination Unit. Project Number: FHD–2021–2437.

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Authors and Affiliations

  1. Chemistry and Chemical Processing Technology, Kocaeli Vocational School, Kocaeli University, Kocaeli, Turkey

    Meryem Pir

  2. Department of Microbiology, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey

    Fatma Budak

  3. Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey

    Kemal Metiner

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Correspondence to Fatma Budak.

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Pir, M., Budak, F. & Metiner, K. In vitro antifungal activity of heterocyclic organoboron compounds against Trichophyton mentagrophytes and Microsporum canis obtained from clinical isolates. Braz J Microbiol 53, 1297–1303 (2022). https://doi.org/10.1007/s42770-022-00777-3

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