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Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources

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Abstract

Three new yeast species, Candida kashinagacola (JCM 15019T = CBS 10903T), C. pseudovanderkliftii (JCM 15025T = CBS 10904T), and C. vanderkliftii (JCM 15029T = CBS 10905T) are described on the basis of comparison of nucleotide sequences of large subunit ribosomal DNA D1/D2 region (LSU rDNA D1/D2). The nearest assigned species of the three new species was Candida llanquihuensis. Candida kashinagacola and C. pseudovanderkliftii differed from C. llanquihuensis by 3.8% nucleotide substitution of the region, while C. vanderkliftii did by 4.4%. Three new species differed in a number of physiological and growth characteristics from any previously assigned species and from one another. A phylogenetic tree based on the sequences of LSU rDNA D1/D2 showed that these new species together with Candida sp. ST-246, Candida sp. JW01-7-11-1-4-y2, Candida sp. BG02-7-20-001A-2-1 and C. llanquihuensis form a clade near Ambrosiozyma species. The new species did not assimilate methanol as a sole source of carbon, which supported the monophyly of these non methanol-assimilating species which are closely related to the methylotrophic yeasts. Candida kashinagacola was frequently isolated from the beetle galleries of Platypus quercivorus in three different host trees (Quercus serrata, Q. laurifolia and Castanopsis cuspidata) located in the sourthern part of Kyoto, Japan, thus indicating that this species may be a primary ambrosia fungus of P. quercivorus. On the other hand, C. pseudovanderkliftii and C. vanderkliftii were isolated only from beetle galleries in Q. laurifolia. Candida vanderkliftii was isolated from beetle gallery of Platypus lewisi as well as those of P. quercivorus. Candida pseudovanderkliftii and C. vanderkliftii are assumed to be auxiliary ambrosia fungi of P. quercivorus.

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Acknowledgements

We thank Dr. M. Kobayashi, Dr. A. Nozaki and technical staffs working for Kamigamo Experimental Station for their help in collecting isolation sources. We also thank Dr. Zaki A. Siddiqui for his help in checking English sentences. We thank Ms. Y. Omoto for her help in isolating yeasts. This research was partially supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (A), no. 18208015, 2006–2008.

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Authors and Affiliations

  1. Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Rikiya Endoh & Kazuyoshi Futai

  2. Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama, 351-0198, Japan

    Rikiya Endoh, Motofumi Suzuki & Yoshimi Benno

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  1. Rikiya Endoh
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  2. Motofumi Suzuki
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  4. Kazuyoshi Futai
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Correspondence to Motofumi Suzuki.

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Endoh, R., Suzuki, M., Benno, Y. et al. Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources. Antonie van Leeuwenhoek 94, 389–402 (2008). https://doi.org/10.1007/s10482-008-9256-9

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