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Modulation of in vitro morphogenesis in nodal segments of Salix tetrasperma Roxb. through the use of TDZ, different media types and culture regimes

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Abstract

A comparative performance of two different media formulations (woody plant medium (WPM) and Murashige and Skoog’s (MS) medium) for their ability to inflict in vitro shoot development in nodal segments of Salix tetrasperma Roxb. has been carried out. Thidiazuron (TDZ) in various concentrations was used as a supplement to the basal media. Media types, TDZ concentrations, exposure duration and culture regimes played an important role in affecting multiple shoot production. WPM supplemented with 2.5 μM TDZ for 4 weeks exposure was found to be the best for maximum (4.53 ± 0.27) shoots production in vitro. Transfer to a secondary medium consisting of 6-benzyladenine (1.0 μM) and α-naphthalene acetic acid (0.5 μM) enhanced the multiplication rate and by the end of 12 weeks, 20.33 ± 0.33 shoots with shoot length, 4.70 ± 0.26 cm were produced on WPM. Rooting of the regenerated shoots was achieved on half strength basal media (either WPM or MS) containing 0.5 μM indole-3-butyric acid. In all the experiments, different growth parameters were scored and WPM was found to be superior to MS medium. The regenerated plantlets were successfully acclimatized in the field with about 81 % survival.

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Abbreviations

BA:

6-benzyladenine

NAA:

α-naphthalene acetic acid

WPM:

Woody plant medium

PGRs:

Plant growth regulators

TDZ:

Thidiazuron

MS:

Murashige and Skoog’s medium

IBA:

Indole-3-butyric acid

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Acknowledgments

Financial assistance to Md Imran Khan in the form of SRF (NET) by Council of Scientific and Industrial Research (CSIR), Govt. of India, New Delhi is gratefully acknowledged. Research support provided by the Department of Science and Technology (DST) and University Grants Commission (UGC) Govt. of India, New Delhi, in the form of DST-FIST (2011) and UGC DRS-I (2009) Programmes is duly acknowledged.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    Md Imran Khan & Mohammad Anis

  2. Department of Plant Production, College of Food and Agriculture Science, King Saud University, PO Box 2460, Riyadh, 11451, Saudi Arabia

    Mohammad Anis

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  1. Md Imran Khan
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  2. Mohammad Anis
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Correspondence to Mohammad Anis.

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Khan, M.I., Anis, M. Modulation of in vitro morphogenesis in nodal segments of Salix tetrasperma Roxb. through the use of TDZ, different media types and culture regimes. Agroforest Syst 86, 95–103 (2012). https://doi.org/10.1007/s10457-012-9512-x

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  • DOI: https://doi.org/10.1007/s10457-012-9512-x

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