Abstract
The kidney is one of the most radiosensitive organs; it is the primary dose-limiting organ in radiotherapies for upper abdominal cancers. The role of mitochondrial redox state in the development and treatment of renal radiation injury, however, remains ill-defined. This study utilizes 3D optical cryo-imaging to quantify renal mitochondrial bioenergetics dysfunction after 13 Gy leg-out partial body irradiation (PBI). Furthermore, the mitigating effects of lisinopril (lisino), an anti-hypertensive angiotensin converting enzyme inhibitor, is assessed in renal radiation-induced injuries. Around day 150 post-irradiation, kidneys are harvested for cryo-imaging. The 3D images of the metabolic indices (NADH, nicotinamide adenine dinucleotide, and FAD, flavin adenine dinucleotide) are acquired, and the mitochondrial redox states of the irradiated and irradiated + lisino kidneys are quantified by calculating the volumetric mean redox ratio (NADH/FAD). PBI oxidized renal mitochondrial redox state by 78%. The kidneys from the irradiated + lisino rats showed mitigation of mitochondrial redox state by 93% compared to the PBI group. The study provides evidence for an altered bioenergetics and energy metabolism in the rat model of irradiation-induced kidney damage. In addition, the results suggest that lisinopril mitigates irradiation damage by attenuating the oxidation of mitochondria leading to increase redox ratio.
Similar content being viewed by others
References
Agarwal, B., R. K. Dash, D. F. Stowe, Z. J. Bosnjak, and A. K. Camara. Isoflurane modulates cardiac mitochondrial bioenergetics by selectively attenuating respiratory complexes. Biochim Biophys Acta Bioenerg 354–365:2014, 1837.
Aldakkak, M., A. K. Camara, J. S. Heisner, M. Yang, and D. F. Stowe. Ranolazine reduces Ca2+ overload and oxidative stress and improves mitochondrial integrity to protect against ischemia reperfusion injury in isolated hearts. Pharmacol. Res. 64:381–392, 2011.
Baskar, R., K. A. Lee, R. Yeo, and K.-W. Yeoh. Cancer and radiation therapy: current advances and future directions. Int J Med Sci 9:193, 2012.
Brandes, R., and D. M. Bers. Increased work in cardiac trabeculae causes decreased mitochondrial NADH fluorescence followed by slow recovery. Biophys. J . 71:1024–1035, 1996.
Burdelya, L. G., V. I. Krivokrysenko, T. C. Tallant, E. Strom, A. S. Gleiberman, D. Gupta, O. V. Kurnasov, F. L. Fort, A. L. Osterman, and J. A. DiDonato. An agonist of toll-like receptor 5 has radioprotective activity in mouse and primate models. Science 320:226–230, 2008.
Camara, A. K., E. J. Lesnefsky, and D. F. Stowe. Potential therapeutic benefits of strategies directed to mitochondria. Antioxid. Redox Signal. 13:279–347, 2010.
Cassady, J. R. Clinical radiation nephropathy. Int. J. Radiat. Oncol. Biol. Phys. 31:1249–1256, 1995.
Cohen, J. J. Is the function of the renal papilla coupled exclusively to an anaerobic pattern of metabolism? Am. J. Physiol. 236:F423–433, 1979.
Cohen, E. P., M. Bedi, A. A. Irving, E. Jacobs, R. Tomic, J. Klein, C. A. Lawton, and J. E. Moulder. Mitigation of late renal and pulmonary injury after hematopoietic stem cell transplantation. Int. J. Radiat. Oncol. Biol. Phys. 83:292–296, 2012.
Cohen, E. P., B. L. Fish, J. D. Imig, and J. E. Moulder. Mitigation of normal tissue radiation injury: evidence from rat radiation nephropathy models. J. Radiat. Oncol. 5:1–8, 2016.
Cowley, A. W., C. Yang, N. N. Zheleznova, A. Staruschenko, T. Kurth, L. Rein, V. Kumar, K. Sadovnikov, A. Dayton, M. Hoffman, R. P. Ryan, M. M. Skelton, F. Salehpour, M. Ranji, and A. Geurts. Evidence of the importance of Nox4 in production of hypertension in dahl salt-sensitive rats. Hypertension 67:440–450, 2016.
Dawson, L. A., B. D. Kavanagh, A. C. Paulino, S. K. Das, M. Miften, X. A. Li, C. Pan, R. K. Ten Haken, and T. E. Schultheiss. Radiation-associated kidney injury. Int. J. Radiat. Oncol. Biol. Phys. 76:S108–S115, 2010.
Epstein, F. H. Oxygen and renal metabolism. Kidney Int. 51:381–385, 1997.
Estabrook, R. W. Fluorometric measurement of reduced pyridine nucleotide in cellular and subcellular particles. Anal. Biochem. 4:231–245, 1962.
Fish, B. L., F. Gao, J. Narayanan, C. Bergom, E. R. Jacobs, E. P. Cohen, J. E. Moulder, C. M. Orschell, and M. Medhora. Combined hydration and antibiotics with lisinopril to mitigate acute and delayed high-dose radiation injuries to multiple organs. Health Phys. 111:410–419, 2016.
Fish, B. L., F. Gao, J. Narayanan, C. Bergom, E. R. Jacobs, E. P. Cohen, J. E. Moulder, C. M. Orschell, and M. Medhora. Combined hydration and antibiotics with lisinopril to mitigate acute and delayed high-dose radiation injuries to multiple organs. Health Phys. 111:410–419, 2016.
Fliedner, T., H. Dorr, and V. Meineke. Multi-organ involvement as a pathogenetic principle of the radiation syndromes: a study involving 110 case histories documented in SEARCH and classified as the bases of haematopoietic indicators of effect. Br. J. Radiol. 1:1–8, 2005.
Gao, F., B. L. Fish, J. E. Moulder, E. R. Jacobs, and M. J. R. Medhora. Enalapril mitigates radiation-induced pneumonitis and pulmonary fibrosis if started 35 days after whole-thorax irradiation. Radiat. Res. 180:546–552, 2013.
Geraci, J., M. Sun, and M. Mariano. Amelioration of radiation nephropathy in rats by postirradiation treatment with dexamethasone and/or captopril. Radiat. Res. 143:58–68, 1995.
Hafer, N., D. Cassatt, A. DiCarlo, N. Ramakrishnan, J. Kaminski, M.-K. Norman, B. Maidment, and R. J. H. Hatchett. NIAID/NIH radiation/nuclear medical countermeasures product research and development program. Health Phys. 98:903–905, 2010.
Hansell, P., W. J. Welch, R. C. Blantz, and F. Palm. Determinants of kidney oxygen consumption and their relationship to tissue oxygen tension in diabetes and hypertension. Clin. Exp. Pharmacol. Physiol. 40:123–137, 2013.
Hur, K. Y., and M. S. Lee. New mechanisms of metformin action: focusing on mitochondria and the gut. J. Diabetes Investig. 6:600–609, 2015.
Jacobs, E. R., J. Narayanan, B. L. Fish, F. Gao, L. M. Harmann, C. Bergom, T. Gasperetti, J. L. Strande, and M. J. H. Medhora. Cardiac remodeling and reversible pulmonary hypertension during pneumonitis in rats after 13-Gy partial-body irradiation with minimal bone marrow sparing: effect of lisinopril. Health Phys. 116(4):558–565, 2019.
Katz, L. A., A. P. Koretsky, and R. S. Balaban. Respiratory control in the glucose perfused heart A 31P NMR and NADH fluorescence study. FEBS Lett. 221:270–276, 1987.
Kharofa, J., E. P. Cohen, R. Tomic, Q. Xiang, and E. Gore. Decreased risk of radiation pneumonitis with incidental concurrent use of angiotensin-converting enzyme inhibitors and thoracic radiation therapy. Int. J. Radiat. Oncol. Biol. Phys. 84:238–243, 2012.
Ki, Y., W. Kim, Y. H. Kim, D. Kim, J. S. Bae, D. Park, H. Jeon, J. H. Lee, J. Lee, and J. Nam. Effect of coenzyme Q10 on radiation nephropathy in rats. J. Korean Med. Sci. 32:757–763, 2017.
la Cour, M. F., S. Mehrvar, J. S. Heisner, M. M. Motlagh, M. Medhora, M. Ranji, and A. K. S. Camara. Optical metabolic imaging of irradiated rat heart exposed to ischemia-reperfusion injury. J. Biomed. Opt. 23:1–9, 2018.
la Cour, M. F., S. Mehrvar, J. Kim, A. Martin, M. A. Zimmerman, J. C. Hong, and M. Ranji. Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries. Biomed. Opt. Express 8:4419–4426, 2017.
Lancaster, S. G., and P. A. J. D. Todd. Lisinopril 35:646–669, 1988.
Lenarczyk, M., E. P. Cohen, B. L. Fish, A. A. Irving, M. Sharma, C. D. Driscoll, and J. E. Moulder. Chronic oxidative stress as a mechanism for radiation nephropathy. Radiat. Res. 171:164–172, 2009.
Lewis, S. A., T. Takimoto, S. Mehrvar, H. Higuchi, A.-L. Doebley, G. Stokes, N. Sheibani, S. Ikeda, M. Ranji, and A. J. P. Ikeda. The effect of Tmem135 overexpression on the mouse heart. PLoS ONE 13:e0201986, 2018.
Liu, Q., G. Grant, and T. Vo-Dinh. Investigation of synchronous fluorescence method in multicomponent analysis in tissue. IEEE J. Sel. Top. Quantum Electron. 16:927–940, 2010.
MacVittie, T. J., A. Bennett, C. Booth, M. Garofalo, G. Tudor, A. Ward, T. Shea-Donohue, D. Gelfond, E. McFarland, and W. Jackson, III. The prolonged gastrointestinal syndrome in rhesus macaques: the relationship between gastrointestinal, hematopoietic, and delayed multi-organ sequelae following acute, potentially lethal, partial-body irradiation. Health Phys. 103:427, 2012.
Medhora, M., F. Gao, T. Gasperetti, J. Narayanan, A. H. Khan, E. R. Jacobs, and B. L. Fish. Delayed effects of acute radiation exposure (Deare) in Juvenile and old rats: mitigation by lisinopril. Health Phys. 116(4):529–545, 2019.
Medhora, M., F. Gao, Q. Wu, R. C. Molthen, E. R. Jacobs, J. E. Moulder, and B. L. Fish. Model development and use of ACE inhibitors for preclinical mitigation of radiation-induced injury to multiple organs. Radiat. Res. 182:545–555, 2014.
Moulder, J. E., E. P. Cohen, and B. L. Fish. Mitigation of experimental radiation nephropathy by renin-equivalent doses of angiotensin converting enzyme inhibitors. Int. J. Radiat. Biol. 90:762–768, 2014.
Moulder, J. E., E. P. Cohen, and B. L. Fish. Mitigation of experimental radiation nephropathy by renin-equivalent doses of angiotensin converting enzyme inhibitors. Int. J. Radiat. Biol. 90:762–768, 2014.
Moulder, J. E., and B. L. Fish. Effect of sequencing on combined toxicity of renal irradiation and cisplatin. NCI Monogr. 6:35–39, 1988.
Nuutinen, E. M. Subcellular origin of the surface fluorescence of reduced nicotinamide nucleotides in the isolated perfused rat heart. Basic Res. Cardiol. 79:49–58, 1984.
Okunieff, P., Y. Chen, D. J. Maguire, and A. K. Huser. Molecular markers of radiation-related normal tissue toxicity. Cancer Metastasis Rev. 27:363–374, 2008.
Robbins, M. E., R. S. Jaenke, T. Bywaters, S. J. Golding, M. Rezvani, E. Whitehouse, and J. W. Hopewell. Sequential evaluation of radiation-induced glomerular ultrastructural changes in the pig kidney. Radiat. Res. 135:351–364, 1993.
Robbins, M., and W. Zhao. Chronic oxidative stress and radiation-induced late normal tissue injury: a review. Int. J. Radiat. Biol. 80:251–259, 2004.
Robbins, M. E., W. Zhao, C. S. Davis, S. Toyokuni, and S. M. Bonsib. Radiation-induced kidney injury: a role for chronic oxidative stress? Micron 33:133–141, 2002.
Sepehr, R., K. Staniszewski, S. Maleki, E. R. Jacobs, S. Audi, and M. Ranji. Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress. J. Biomed. Opt. 17:0460101–0460107, 2012.
Soltoff, S. P. ATP and the regulation of renal cell function. Annu. Rev. Physiol. 48:9–31, 1986.
Sosunov, S. A., X. Ameer, Z. V. Niatsetskaya, I. Utkina-Sosunova, V. I. Ratner, and V. S. Ten. Isoflurane anesthesia initiated at the onset of reperfusion attenuates oxidative and hypoxic-ischemic brain injury. PLoS ONE 10:e0120456, 2015.
Stowe, D. F., and A. K. Camara. Mitochondrial reactive oxygen species production in excitable cells: modulators of mitochondrial and cell function. Antioxid. Redox Signal. 11:1373–1414, 2009.
Vidt, D. G., E. L. Bravo, and F. M. N. J. E. J. O. M. Fouad. Captopri 306:214–219, 1982.
Watanabe Nemoto, M., K. Isobe, G. Togasaki, A. Kanazawa, M. Kurokawa, M. Saito, R. Harada, H. Kobayashi, H. Ito, and T. Uno. Delayed renal dysfunction after total body irradiation in pediatric malignancies. J. Radiat. Res. 55:996–1001, 2014.
Williams, J. P., S. L. Brown, G. E. Georges, M. Hauer-Jensen, R. P. Hill, A. K. Huser, D. G. Kirsch, T. J. MacVittie, K. A. Mason, M. M. Medhora, J. E. Moulder, P. Okunieff, M. F. Otterson, M. E. Robbins, J. B. Smathers, and W. H. McBride. Animal Models for Medical Countermeasures to Radiation Exposure. Radiat. Res. 173:557–578, 2010.
Acknowledgment
We thank Brian Fish, Jayashree Narayanan, Tracy Gasperetti, and James Heisner for excellent animal care, irradiation, dosimetry and tissue harvesting. We also are thankful for funding support from UWM RGI 101X290 and 101 × 397, NIAID R01-101898, U01-107305, U01AI33594, Department of Radiation Oncology, Cardiovascular and Cancer Centers at MCW and NIH grant R01HL116530.
Conflict of interest
None Declared.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Associate Editor Dan Elson oversaw the review of this article.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Mehrvar, S., la Cour, M.F., Medhora, M. et al. Optical Metabolic Imaging for Assessment of Radiation-Induced Injury to Rat Kidney and Mitigation by Lisinopril. Ann Biomed Eng 47, 1564–1574 (2019). https://doi.org/10.1007/s10439-019-02255-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10439-019-02255-8