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Subcellular origin of the surface fluorescence of reduced nicotinamide nucleotides in the isolated perfused rat heart

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Summary

Surface fluorometric measurements and indicator metabolite determinations in the isolated perfused rat heart showed that the NADH+NADPH fluorescence of the intact tissue originates largely from the mitochondria. The redox potential of the lactate dehydrogenase system calculated from the endogenous lactate/pyruvate ratios was closely similar to that of the glycerol-3-phosphate dehydrogenase system calculated from the concentrations of glycerol-3-phosphate and dihydroxyacetone phosphate in the tissue. Thus, in contrast to the liver, the cytosolic redox state of the NADH/NAD+ system in isolated perfused heart oxidizing external glucose or fatty acid is not amenable to optical monitoring, but can be assessed from the state of the lactate dehydrogenase or glycerol-3-phosphate, dehydrogenase systems.

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  1. E. M. Nuutinen M.D.

    Present address: Department of Medical Biochemistry, University of Oulu, Kajaanintie 52 A, SF-90220, Oulu, Finland

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    Nuutinen, E.M. Subcellular origin of the surface fluorescence of reduced nicotinamide nucleotides in the isolated perfused rat heart. Basic Res Cardiol 79, 49–58 (1984). https://doi.org/10.1007/BF01935806

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    • DOI: https://doi.org/10.1007/BF01935806

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