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Assessment of cross-type optical particle separation system

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Abstract

This paper describes the optical and hydrodynamic characteristics of particle motion in a cross-type optical particle separator. The retention distance modulated by the optical force on a particle was measured in three dimensions for various vertical and horizontal positions via μ-defocusing digital particle image velocimetry. The experimental data showed that the actual retention distance was smaller than the predicted retention distance under the assumption that the approaching velocity was constant through the cross-section of a microfluidic channel. The retention distance was shown to increase as the injection position of the particle shifted toward the channel side wall at a given vertical position due to a higher residence time within the region of influence of the laser beam. In contrast, the retention distance decreased as the injection position shifted toward the channel top/bottom walls at a given horizontal position. A theoretical modeling study was conducted to support and interpret the experimental measurements. The resolution of the particle separation procedure, which did not require adjusting the flow rate, laser power, or working fluid properties, was studied.

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References

  • Ashkin A (1970) Acceleration and trapping of particles by radiation pressure. Phys Rev Lett 24:156–159

    Article  Google Scholar 

  • Bruus H (2008) Theoretical microfluidics. Oxford University Press, New York

    Google Scholar 

  • Carlo DD, Irimia D, Tompkins RG, Toner M (2007) Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci USA 104:18892–18897

    Article  Google Scholar 

  • Chang C–C, Huang Z-X, Yang R-J (2007) Three-dimensional hydrodynamic focusing in two-layer polydimethylsiloxane (PDMS) microchannels. J Micromech Microeng 17:1479–1486

    Article  Google Scholar 

  • Dholakia K, Reece P, Gu M (2008) Optical micromanipulation. Chem Soc Rev 37:42–55

    Article  Google Scholar 

  • Fuerstman MJ, Lai A, Thurlow ME, Shevkoplyas SS, Stone HA, Whitesides GM (2007) The pressure drop along rectangular microchannels containing bubbles. Lab Chip 7:1479–1489

    Article  Google Scholar 

  • Gomez FA (2008) Biological applications of microfluidics. Wiley, New Jersey

    Google Scholar 

  • Hakem IF, Leech AM, Johnson JD, Donahue SJ, Walker JP, Bockstaller MR (2010) Understanding ligand distribution in modified particle and particle like systems. J Am Chem Soc 132:16593–16598

    Article  Google Scholar 

  • Hart SJ, Terray AV (2003) Refractive-index-driven separation of colloidal polymer particles using optical chromatography. Appl Phys Lett 83:5316–5318

    Article  Google Scholar 

  • Helmbrecht C, Niessner R, Haisch C (2007) Photophoretic velocimetry for colloid characterization and separation in a cross-flow setup. Anal Chem 79:7097–7103

    Article  Google Scholar 

  • Hoi S-K, Udalagama C, Sow C-H, Watt F, Bettiol AA (2009) Microfluidic sorting system based on optical force switching. Appl Phys B 97:859–865

    Article  Google Scholar 

  • Hoi S-K, Hu Z-B, Yan Y, Sow C-H, Bettiol AA (2010) A microfluidic device with integrated optics for microparticle switching. Appl Phys Lett 97:183501

    Google Scholar 

  • Hsu H-Y, Ohta AT, Chiou P-Y, Jamshidi A, Neale SL, Wu MC (2010) Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media. Lab Chip 10:165–172

    Article  Google Scholar 

  • Kim SB, Kim SS (2006) Radiation forces on spheres in loosely focused Gaussian beam: ray-optics regime. J Opt Soc Am B 23:897–903

    Article  Google Scholar 

  • Kim YW, Yoo JY (2009) Axisymmetric flow focusing of particles in a single microchannel. Lab Chip 9:1043–1045

    Article  Google Scholar 

  • Kim SB, Kim JH, Kim SS (2006) Theoretical development of in situ optical particle separator: cross-type optical particle chromatography. Appl Opt 45:6919–6924

    Article  Google Scholar 

  • Kim SB, Jung E, Sung HJ, Kim SS (2008a) Optical mobility in cross-type optical particle separation. Appl Phys Lett 93:044103

    Article  Google Scholar 

  • Kim SB, Yoon SY, Sung HJ, Kim SS (2008b) Cross-type optical particle separation in a microchannel. Anal Chem 80:2628–2630

    Article  Google Scholar 

  • Kinoshita H, Kaneda S, Fujii T, Oshima M (2007) Three-dimensional measurement and visualization of internal flow of a moving droplet using confocal micro-PIV. Lab Chip 7:338–346

    Article  Google Scholar 

  • Kuhn S, Measor P, Lunt EJ, Philips BS, Deamer DW, Hawkins AR, Schmidt H (2009) Loss-based optical trap for on-chip particle analysis. Lab Chip 9:2212–2216

    Article  Google Scholar 

  • Ladavac K, Kasza K, Grier DG (2004) Sorting mesoscopic objects with periodic potential landscapes: optical fractionation. Phys Rev E 70:010901

    Article  Google Scholar 

  • Lee KH, Kim SB, Lee KS, Sung HJ (2011) Enhancement by optical force of separation in pinched flow fractionation. Lab Chip 11:354–357

    Article  Google Scholar 

  • MacDonald MP, Spalding GC, Dholakia K (2003) Microfluidic sorting in an optical lattice. Nature 426:421–424

    Article  Google Scholar 

  • Mao X, Lin S-CS, Dong C, Huang TJ (2009) Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. Lab Chip 9:1583–1589

    Article  Google Scholar 

  • Maruyama H, Kotani K, Masuda T, Honda A, Takahata T, Arai F (2011) Nanomanipulation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip. Microfluid Nanofluid 10:1109–1117

    Article  Google Scholar 

  • Ohshima H, Kondo T (1989) Approximate analytical expression for the electrophoretic mobility of colloidal particles with surface-charge layers. J Colloid Interface Sci 130:281–282

    Article  Google Scholar 

  • Pamme N, Manz A (2004) On-chip free-flow magnetophoresis: continuous flow separation of magnetic particles and agglomerates. Anal Chem 76:7250–7256

    Article  Google Scholar 

  • Park JS, Kihm KD (2006) Three-dimensional micro-PTV using deconvolution microscopy. Exp Fluids 40:491–499

    Article  Google Scholar 

  • Psaltis D, Quake SR, Yang C (2006) Developing optofluidic technology through the fusion of microfluidics and optics. Nature 442:381–386

    Article  Google Scholar 

  • Tsai C-H, Hou H-H, Fu L-M (2008) An optimal three-dimensional focusing technique for micro-flow cytometers. Microfluid Nanofluid 5:827–836

    Article  Google Scholar 

  • Wang MM, Tu E, Raymond DE, Yang JM, Zhang H, Hagen N, Dees B, Mercer WM, Forster AH, Kariv I, Marchand PJ, Butler WF (2005) Microfluidic sorting of mammalian cells by optical force switching. Nat Biotechnol 23:83–87

    Article  Google Scholar 

  • Wu M, Robert JW, Buckley M (2005) Three-dimensional fluorescent particle tracking at micron-scale using a single camera. Exp Fluids 38:461–465

    Article  Google Scholar 

  • Yamada M, Nakashima M, Seki M (2004) Pinched flow fractionation: continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel. Anal Chem 76:5465–5471

    Article  Google Scholar 

  • Yang AHJ, Moore SD, Schmidt BS, Klug M, Lipson M, Erickson D (2009) Optical manipulation of nanoparticles and biomolecules in sub-wavelength slot waveguides. Nature 457:71–75

    Article  Google Scholar 

  • Yoon SY, Kim KC (2006) 3D particle position and 3D velocity field measurement in a microvolume via the defocusing concept. Meas Sci Technol 17:2897–2905

    Article  Google Scholar 

  • Yoon SY, Kihm KD, Kim KC (2011) Correlation of fluid refractive index with calibration coefficient for micro-defocusing digital particle image velocimetry. Meas Sci Technol 22:037001

    Article  Google Scholar 

  • Yu C, Vykoukal J, Vykoukal DM, Schwartz JA, Shi L, Gascoyne PRC (2005) A three-dimensional dielectrophoretic particle focusing channel for microcytometry applications. J Microelectromech Syst 14:480–487

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the Creative Research Initiatives (No. 2011-0000423) program of the National Research Foundation of Korea.

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Authors and Affiliations

  1. Department of Mechanical Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Korea

    Kang Soo Lee, Sang Youl Yoon, Sang Bok Kim, Kyung Heon Lee, Hyung Jin Sung & Sang Soo Kim

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  1. Kang Soo Lee
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  2. Sang Youl Yoon
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  3. Sang Bok Kim
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  4. Kyung Heon Lee
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  5. Hyung Jin Sung
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  6. Sang Soo Kim
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Correspondence to Hyung Jin Sung or Sang Soo Kim.

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Lee, K.S., Yoon, S.Y., Kim, S.B. et al. Assessment of cross-type optical particle separation system. Microfluid Nanofluid 13, 9–17 (2012). https://doi.org/10.1007/s10404-012-0935-5

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  • DOI: https://doi.org/10.1007/s10404-012-0935-5

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